生物加工过程2019，Vol.17Issue(2)：144-151,8.DOI:10.3969/j.issn.1672-3678.2019.02.005
磷脂酰胆碱特异性和磷脂酰肌醇特异性磷脂酶C酶学性质及其在油脂脱胶中的应用
Characterization of phosphatidylcholine- and phosphatidylinositol-specific phospholipase C and its application in oil degumming
邹权 1陈圣 1严明1
作者信息
- 1. 南京工业大学 生物与制药工程学院,江苏 南京 211800
- 折叠
摘要
Abstract
We transported two phospholipase C genes to Bacillus sublitis WB800 to express phosphatidylcholine, phosphatidylethanolamine-and phosphatidylinositol-specific properties by genetic engineering methods.Then the two recombinant proteins were identified, characterized and evaluated on their degumming efficiency.Both enzymes exhibited high temperature (retaining 60％ catalytic activity, 30-45 ℃ for 2 h) and pH stability (retaining 60％ activity, pH 6.0-7.0 for 2 h).Besides, the metal ions (Zn2+ and Mg2+) could increase the catalytic activity of PC-PLC whereas the activity of PI-PLC increased by Ca2+.The oil degumming results showed that the optimal degumming condition of PC-PLC was 50 ℃, pH 6.0, enzyme dosage of 80 mg/kg oil and reaction time of 2.5 h.The optimal degumming condition of PI-PLC was 45 ℃, pH 7.0, enzyme dosage of 60 mg/kg oil, reaction time of 2.5 h.The degumming effects of combining PC-PLC and PI-PLC to treat crude soybean oil, phosphorus content decreased from 531 mg/kg to 4.1 mg/kg and the yield of DAG increased 0.95％.关键词
PC、PE特异性磷脂酶C/PI特异性磷脂酶C/酶法脱胶Key words
PC and PE specific phospholipase C/PI specific phospholipase C/enzymatic degumming分类
生物科学引用本文复制引用
邹权,陈圣,严明..磷脂酰胆碱特异性和磷脂酰肌醇特异性磷脂酶C酶学性质及其在油脂脱胶中的应用[J].生物加工过程,2019,17(2):144-151,8.
