生物加工过程2019，Vol.17Issue(2)：177-183,7.DOI:10.3969/j.issn.1672-3678.2019.02.010
分枝杆菌LY-1转化植物甾醇产9α羟基雄烯二酮的发酵工艺优化
Fermentation process of Mycobacterium sp. LY-1 for the transformation of phytosterol to 9α-hydroxy-4-androstenedione
摘要
Abstract
9α-Hydroxy-4-androstene-3, 17-dione (9α-OH-AD) is an important precursor to produce glucocorticoid drugs. To improve the yield of 9α-OH-AD, Mycobacterium sp. LY-1 was used as the wild strain. To study the effects of different carbon sources, nitrogen source, phosphate and inorganic salts on the concentration of 9α-OH-AD, we optimized the medium by orthogonal experiments.The optimized medium was composed as (g/L):corn steep liquor 30, KNO3 4.0, NaH2PO4 1.2, FeSO4 0.075, and CaCl2 0.10. Under this condition, the yield of 9α-OH-AD reached 43.9％-44.9％ when the concentration of phytosterol was 15 g/L. Then, the conditions of culture were optimized through single factors experiments. The suitable culture conditions were determined as:pH 8.0, 2％ inoculum concentration and 30 ℃. Under these conditions, the yield of 9α-OH-AD reached 46.2％-47.0％, increased by 22.1％ compared with the control, and the concentration of 9α-OH-AD was 5.21 g/L. It has a potential industrial application.关键词
Mycobacterium sp./植物甾醇/9α-OH-AD/正交优化Key words
Mycobacterium sp./phytosterol/9α-hydroxy-4-androstene-3, 17-dione/orthogonal optimization分类
生物科学引用本文复制引用
王向栋,李会,陈志蔚,蔡兆培,史劲松,许正宏..分枝杆菌LY-1转化植物甾醇产9α羟基雄烯二酮的发酵工艺优化[J].生物加工过程,2019,17(2):177-183,7.基金项目
江苏省普通高校学术学位研究生科研创新计划(KYLX16_0826) （KYLX16_0826）
