山西农业大学学报（自然科学版）2019，Vol.39Issue(3)：1-6,6.DOI:10.13842/j.cnki.issn1671-8151.201901032
外源MeJ A对低温下紫苏脂肪酸合成及FAD 3基因表达的调控
Effects of exogenous MeJA on the regulation of fatty acid synthesis and FAD 3 gene expression in Peril-la frutescens under low temperature
摘要
Abstract
[Obj ectives]Methyl jasmonate (MeJA)is a natural plant growth regulator which profoundly modifies plant stress response.In this paper,the effect of exogenous MeJA on the cold resistance of perilla seedlings was investiga-ted,and aimed to provide a theoretical basis for the study of the resistance mechanism of perilla.[Methods]The perilla variety,"Jinzisu 1″,was employed as the experimental material,and perilla seedlings were treated with 0.025 mmol· L-1 MeJA for 0,3 ,6 ,9 ,1 2 ,and 24 h at 4℃.The ones without MeJA treatment served as the control group at the same temperature.The fatty acid composition and content,relative electrical conductivity,the content of malondialde-hyde,and relative expression levels of the omega-3 fatty acid dehydrogenase gene (FAD 3 )in the seedling leaves of each treatment were measured.[Results]The content of unsaturated fatty acid C18:3 was increased dramatically when plants were treated with MeJA at 4℃,and it was peaked at 6 h post treatment with 6.9 6 times higher concentration than that of the control.The total fatty acid content in perilla leaves was increased significantly in all different treat-ments.The relative electrical conductivity and MDA content were decreased significantly with the prolongation of low temperature stress plus MeJA treatments.The relative expression level of FAD3 gene of MeJA treatment was peaked at 3 h post treatment,which was 1 5 .9 times higher than that of the control.[Conclusion]Under the condition of exog-enous MeJA combined with low temperature,high expression of FAD3 gene in perilla seedlings was induced and the content of unsaturated fatty acids was increased.Besides,the perilla cell membrane integrity could be better maintained and the cold resistance of seedlings was enhanced.关键词
紫苏/茉莉酸甲酯/低温胁迫/脂肪酸/FAD3 基因Key words
Perilla frutescens/Methyl jasmonate/Low temperature stress/Fatty acid/FAD 3 gene分类
农业科技引用本文复制引用
王计平,梁倩,周雅莉,李润植,薛丽芳,郝晓娟..外源MeJ A对低温下紫苏脂肪酸合成及FAD 3基因表达的调控[J].山西农业大学学报（自然科学版）,2019,39(3):1-6,6.基金项目
国家自然科学基金(30770239) （30770239）
山西省自然科学基金(2009011048) （2009011048）
