山西农业大学学报（自然科学版）2019，Vol.39Issue(3)：14-22,9.DOI:10.13842/j.cnki.issn1671-8151.201811061
响应面法优化黄芪三种活性成分的闪式提取工艺
Determination of optimal flash extraction condition for three active compounds of Astragalus membra-naceus using response surface methodology
摘要
Abstract
[Obj ectives]The aim of current study was to determine the optimal technological conditions for the flash ex-traction of three main active components in Astragalus membranaceus .[Methods]The contents of flavonoids,poly-saccharides,and saponins were determined by ultraviolet,aluminum nitrate-sodium nitrite method,phenol-sulfuric acid method,and vanilla-sulfuric acid method,respectively.The yield of each component was used as the indexes to optimize the extraction process parameters by response surface methodology,and the regression models were obtained. [Results]The results showed that the developed regression model precisely reflected the relationship between the yield of flavonoids,polysaccharides,and saponins and extraction conditions.The optimal technological conditions were de-termined as following:the higher concentration of flavonoids was obtained when ethanol concentration at 85 %,solid-liquid ratio at 1 :25 ,extraction time at 60 s,and extraction voltage at 120 V;the highest concentration of polysaccha-rides was achieved when ethanol concentration at 2.5 %,solid-liquid ratio at 1∶1 5,extraction time at 105 s,and ex-traction voltage at 1 15 V;while the greatest concentration of saponin was reached when ethanol concentration at 70 %, solid-liquid ratio at 1∶30,extraction time at 120 s,and extraction voltage at 1 10 V.[Conclusion]Under the mentioned conditions,the yield of flavonoids,polysaccharides,and saponins were 0.663%,0.965%,and 1.126%,respectively. The difference between the predicted and measured values of the regression models were less than 0.05%.The three re-gression equations fitted well to the actual situation and the optimized process was reliable.关键词
黄芪活性成分/响应面法/闪式提取技术Key words
Active components of Astragalus membranaceus/Response surface methodology/Flash extraction technology分类
医药卫生引用本文复制引用
王梦茹,雷振宏,梁华,李文倩,张莹,梁建萍,赵建平,柴智..响应面法优化黄芪三种活性成分的闪式提取工艺[J].山西农业大学学报（自然科学版）,2019,39(3):14-22,9.基金项目
山西省重点研发计划重点项目(201703D211001-04-05) （201703D211001-04-05）
山西农业大学青年拔尖创新人才支持计划(BJRC201601) （BJRC201601）
山西省回国留学人员科研资助项目(2015-065) （2015-065）
山西省农业科技成果转化和推广示范项目(SXNKTG201714) （SXNKTG201714）
