山西农业大学学报（自然科学版）2019，Vol.39Issue(3)：23-28,6.DOI:10.13842/j.cnki.issn1671-8151.201812008
萱草属植物短缩茎组织培养及植株再生
Tissue culture and plant regeneration with dwarf stem of Hemerocallis plants
摘要
Abstract
[Obj ective]This study was aimed to construct a rapid propagation system with the dwarf stem of the Hemero-callis by inducing the adventitious bud pathway into plants.[Methods]Datong Huanghua was selected as the research material,and the effects of 6-BA and NAA combination at 10 different concentrations on the induction rate of adventi-tious bud,adventitious bud proliferation,and rooting rate of Hemerocallis were studied at different sampling time.[Re-sults]The results showed that the best sampling time for short-stem culture of'Datong Huanghua'was the germination stage (February to late March).The optimal medium for adventitious bud induction was the mixture of MS plus 1 .0 mg/L 6-BA and 0.1 mg/L NAA which produced the highest induction rate at 88.89%,with the highest induction coefficient at 6 .6 7 .The adventitious bud proliferation medium was determined as MS combined with 2 .0 mg/L 6-BA and 0.5 mg/L NAA.When the adventitious bud was at 3~5 cm length,and was inoculated into the rooting medium of MS+0.2 5 mg/L NAA,the highest root number was measured as 4.33 per plant.When 14 short stems of the genus Hemerocallis were inoculated into the adventitious bud induction medium as described previously,adventitious buds were induced in 1 2 out of 14 testing material.[Conclusion]In this study,the tissue culture and plant regeneration system of the dwarf stem of Hemerocallis were developed,and the explant selection of the tissue culture of Hemerocallis was extended,which pro-vided technical support for the annual tissue culture of Hemerocallis .关键词
萱草属(Hemerocallis spp .) /短缩茎/不定芽/组织培养Key words
Hemerocallis spp/Dwarf stem/Adventitious bud/Tissue culture分类
农业科技引用本文复制引用
王荣梅,褚焕宁,王金耀,李森,亢秀萍..萱草属植物短缩茎组织培养及植株再生[J].山西农业大学学报（自然科学版）,2019,39(3):23-28,6.基金项目
山西省重点研发计划重点项目(201703D211001-04-04) （201703D211001-04-04）
山西省重点研发计划项目(201703D221028-4) （201703D221028-4）
