实用医学杂志2019，Vol.35Issue(3)：375-380,6.DOI:10.3969/j.issn.1006-5725.2019.03.009
大鼠滑膜间充质干细胞的鉴定及定向分化
Identification of rat synovial mesenchymalstem cells and experimental study on their directional differentiation
摘要
Abstract
Objective To isolate, culture and expand SD rat synovial mesenchymal stem cells in vitro and identify their potential multilineage differentiation. Methods Traumatic osteoarthritis models were made in SD rats. Synovial tissue was collected under aseptic conditions. The synovial tissue was digested with type I collagenase to isolate synovial mesenchymal stem cells. Cells were purified by limiting dilution of the monoclonal culture and expanded in vitro. After the third passage of culture, growth curve determination, adipogenesis induction, osteoinduction and chondrogenesis induction were performed. Real-time qPCR and Western blot were used to detect the expression of proteoglycan and type Ⅱ collagen after chondrogenic differentiation. Results In the present study, rat synovial mesenchymal stem cells exhibited a homogeneous, fibroblast-like, spindle-shaped morphology after passage in vitro. Synovial mesenchymal stem cells were subjected to adipogenesis induction, osteoinduction and chondrogenesis induction and being identified. Under the influence of inflammatory factors, mRNA of type Ⅱ collagen and proteoglycans was positively expressed in differentiated cells induced by chondrocytes using real-time qPCR and Western blot. Conclusion Rat-derived synovial mesenchymal stem cells still have good chondrogenic potential in inflammatory environment.关键词
滑膜间充质干细胞/成软骨分化潜能/骨关节炎/炎症因子Key words
synovial mesenchymal stem cells/chondrogenic potential/osteoarthritis/inflammatory factors引用本文复制引用
王兆飞,王炳,周渊,宋登新,何小文,曹云,王健..大鼠滑膜间充质干细胞的鉴定及定向分化[J].实用医学杂志,2019,35(3):375-380,6.基金项目
上海市卫生和计划生育委员会科研课题(编号:20174Y0240) （编号:20174Y0240）
上海市宝山区科学技术委员会科技创新专项资金项目(编号:17-E-1) （编号:17-E-1）
