实用医学杂志2019，Vol.35Issue(3)：381-383,387,4.DOI:10.3969/j.issn.1006-5725.2019.03.010
缺氧对肺癌细胞系A-549表达缺氧诱导因子1α和2α的影响
Effects of hypoxia on expression of hypoxia inducible factors 1α and 2α in human lung cancer cell line A-549
摘要
Abstract
Objective To investigate the changes of hypoxia-inducible factor (HIF-1α, HIF-2α) expression level in lung cancer A-549 cells under normoxic conditions, different hypoxia durations, and different oxygen concentrations. Methods A549 cells were divided into normoxic group, time control group, and oxygen concentration control group. Western blot was used to detect the expression of HIF-1α and HIF-2α in A-549 cells.Results The expression of HIF-1α and HIF-2α protein were lower under normoxia and significantly increased under hypoxic conditions. The difference was statistically significant. The lower the oxygen concentration, the more HIF-1α and HIF-2α protein expression levels were. The differences between high and high were statistically significant. The expression of HIF-1α protein increased at 2 h after hypoxia, peaked at 8 h, appeared plateau at 8 to 16 h, and decreased at 32 h, with a statistically significant difference. HIF-2α proteins gradually increased with prolonged hypoxia. Conclusions Under hypoxic conditions, the expression of HIF-1α and HIF-2α are increased, and the expression of HIF-2α has a time-dependent pattern, which may have more important biological significance.关键词
肺癌/A549/HIF-1α/HIF-2αKey words
lung cancer/A549/HIF-1α/HIF-2α引用本文复制引用
冯永海,李宏云,苗少一,施小山,赵文飞..缺氧对肺癌细胞系A-549表达缺氧诱导因子1α和2α的影响[J].实用医学杂志,2019,35(3):381-383,387,4.基金项目
河南省科技厅重点项目(编号:162102310133) （编号:162102310133）
