特产研究2019，Vol.41Issue(1)：1-4,4.DOI:10.16720/j.cnki.tcyj.2019.01.001
西洋参果多糖的纯化及DPPH自由基清除活性研究
Research on Purification and DPPH free Radicals Scavenging Activity of Polysaccharide from Panax Quinquefolius Berry
摘要
Abstract
Extracted and purified polysaccharide fractions from Panax quinquefolius berry, and DPPH radicals scavenging activity was carried on.Polysaccharide fractions (WQBP) from P.quinquefolius berry was dealed with water-extraction, ethanol-precipetation and ion-exchange chromatography, then one neutral (WQBP-N) and one acidic fraction (WQBP-A) were obtained.The monosaccharide composition, molecular weight distribution and DPPH free radicals scavenging activity were analyzed.WQBP is mainly composed of rhamnose, galacturonic acid, galactose, arabinose and glucose. WQBP-N is composed of galactose, arabinose, glucose and mannose with a ratio of63.9:19.3:15.2:1.5; WQBP-A is mainly composed by rhamnose, galacturonic acid, galactose, arabinose and glucose with a ratio of13.1:13.2:46.4:19.0:5.8.WQBP has more significant DPPH free radicals scavenging activity than neutral and acidic fraction, which has a concentration dependant tendency.WQBP has potential antioxidant activity in vitro, and the results above could provide theory basis for the further application and developments of the P.quinquefolius berry polysaccharide.关键词
西洋参果/多糖/纯化/1, 1-二苯基-2-二硝基苯肼Key words
Panax quinquefolius berry/polysaccharide/purification/DPPH分类
医药卫生引用本文复制引用
李珊珊,祁玉丽,曲迪,张磊,孙印石..西洋参果多糖的纯化及DPPH自由基清除活性研究[J].特产研究,2019,41(1):1-4,4.基金项目
吉林省科技发展计划医药产业发展引导资金项目(20180311037YY) （20180311037YY）
吉林省科技创新人才计划青年科研基金项目(20160520048JH) （20160520048JH）
