特产研究2019，Vol.41Issue(1)：22-25,4.DOI:10.16720/j.cnki.tcyj.2019.01.006
水貂犬瘟热活疫苗与水貂细小病毒性肠炎灭活疫苗制备及其联合免疫效果研究
Production and Combined Immune Response of Mink Canine Distemper Vaccine Live and Mink Enteritis Virus Vaccine Inactivated
罗国良 1张洪亮 2王振军 1冯二凯 1易立 1郭利 1程悦宁 1程世鹏 1王守富 3王宏正2
作者信息
- 1. 中国农业科学院特产研究所,长春 130112
- 2. 长春西诺生物科技有限公司,长春 130012
- 3. 吉林农业大学,长春 130118
- 折叠
摘要
Abstract
The study prepared 3 batches of mink canine distemper live vaccine and mink parvovirus inactivated vaccine respectively. The results of semi-finished products and finished products of vaccine showed that the two vaccines were qualified and the production process was stable. Vero cells were inoculated after the combination of two vaccines by the mink parvovirus enteritis inactivated vaccine to dilute the live vaccine of the canine distemper. The results indicated that the virus contents of live vaccine of the canine distemper which was diluted by the mink parvovirus enteritis inactivated vaccine (trial group) at 25℃, 5 h was not significantly different compared to the canine distemper live vaccine which was diluted by the special diluent (control group). The combined vaccine immunized 10 minks, canine distemper vaccine and enteritis inactivated vaccine were immunized 10 minks as the control group, and the antibody levels of the three groups were determined until 21 days after immunization. The SN against CDV and the HI against MEV of the combined vaccine were not significantly different from that of the corresponding single vaccine, respectively. This study indicated that the combined vaccine has the same immune effect as the single vaccine. This trial establish basis for further studying the combined vaccine of mink canine distemper vaccine and mink parvovirus inactivated vaccine.关键词
水貂犬瘟热活疫苗/水貂细小病毒性肠炎灭活疫苗/联合免疫Key words
Mink canine distemper vaccine live/Mink enteritis virus inactivated vaccine/combined immunization分类
农业科技引用本文复制引用
罗国良,张洪亮,王振军,冯二凯,易立,郭利,程悦宁,程世鹏,王守富,王宏正..水貂犬瘟热活疫苗与水貂细小病毒性肠炎灭活疫苗制备及其联合免疫效果研究[J].特产研究,2019,41(1):22-25,4.
