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不同产地、生长年限、采收期猪苓中三种有效成分的含量分析

郭宁 武泽宇 王建刚 孙印石 王弘

特产研究2019,Vol.41Issue(1):72-77,94,7.
特产研究2019,Vol.41Issue(1):72-77,94,7.DOI:10.16720/j.cnki.tcyj.2019.01.016

不同产地、生长年限、采收期猪苓中三种有效成分的含量分析

Analysis of Three Effective Components of Polyporus Umbellatus in Different Regions, Different Growth Periods and Different Harvest Periods

郭宁 1武泽宇 1王建刚 1孙印石 2王弘1

作者信息

  • 1. 北京大学药学院,北京 100191
  • 2. 中国农业科学院特产研究所,长春 130112
  • 折叠

摘要

Abstract

The contents of ergosterol, ergosterone-4, 6, 8 (14) , 22-tetraen-3-one and polysaccharide of Polyporus umbellatus in different growth periods and different harvest periods were determined. The best regions, the best growth period and the best harvest period were obtained according to the difference in the content of different components. Ergosterol and ergosterone-4, 6, 8 (14) , 22-tetraen-3-one were determined by HPLC, and polysaccharides in P. umbellatus were determined by UV-vis, glucose as reference substance. The results showed that there were some differences in the contents of ergosterol, ergosterone-4, 6, 8 (14) , 22-tetraen-3-one and polysaccharides in different regions. The highest ergosterol of 1.41 mg/g is from Lincheng, Hebei. The highest ergosterone-4, 6, 8 (14) , 22-tetraen-3-one of 18.87 (g/g is from Luxi, Yunnan. The highest polysaccharides of 4.99% is from Jilin. The contents of three components of P. umbellatus in Shaanxi are generally higher than those in other producing areas. In the same area (Lueyang, Shaanxi) , ergosterol, ergosterone-4, 6, 8 (14) , 22-tetraen-3-one and polysaccharides in P. umbellatus changed with the growth of the growth period. For the same growth period, the harvest time of P. umbellatus is appropriate in spring. This method is simple, sensitive and accurate. It provides scientific basis for finding the best place of regions, the best growth period and the best harvest period.

关键词

猪苓/高效液相色谱/麦角甾醇/麦角甾-4, 6, 8 (14) , 22-四烯-3-酮/总多糖

Key words

Polyporus umbellatus/HPLC/ergosterol/ergosterone-4, 6, 8 (14) , 22-tetraen-3-one/polysaccharide

分类

医药卫生

引用本文复制引用

郭宁,武泽宇,王建刚,孙印石,王弘..不同产地、生长年限、采收期猪苓中三种有效成分的含量分析[J].特产研究,2019,41(1):72-77,94,7.

基金项目

国家发改委新兴产业重大工程包中药标准化项目〔ZYBZH-GD-13(ZYY-2017-159)〕 (ZYY-2017-159)

特产研究

OACSTPCD

1001-4721

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