特产研究2019，Vol.41Issue(1)：120-123,4.DOI:10.16720/j.cnki.tcyj.2019.01.026
数字PCR技术研究进展
Research Progress of Digital PCR Technology
摘要
Abstract
Digital PCR is an emerging technology for quantitative analysis of nucleic acids. The technique is to distribute a PCR reaction containing a nucleic acid template into a large number of reaction units for nucleic acid amplification. After completion of the reaction, using detectable fluorescence for statistical counts to quantify nucleic acid concentration in a sample. However Digital PCR does not require the establishment of a standard curve and has a good application prospect. This article includes digital PCR principles, classification, detection methods, risks and advantages, and application prospects.关键词
数字聚合酶链式反应Key words
Digital PCR分类
生物科学引用本文复制引用
李智杰,刘占悝,李健友,刘泽余,郭利..数字PCR技术研究进展[J].特产研究,2019,41(1):120-123,4.基金项目
国家重点研发计划(2016YFD0500907) （2016YFD0500907）
吉林省科技厅产业技术创新战略联盟项目(20170309002NY) （20170309002NY）
