天津农业科学2019，Vol.25Issue(2)：7-11,5.DOI:10.3969/j.issn.1006-6500.2019.02.002
水蒸气蒸馏法提取八角茴香油及其抑菌活性研究
Extraction of Star Anise Oil by Hydro-distillation and Its Antibacterial Activity
摘要
Abstract
In order to optimize the extraction process of star anise oil and develop it as a plant-derived antibacterial agent for agricultural and food preservation, hydro-distillation method was carried out to extract star anise oil.According to the single factor test, using the extraction rate as an evaluation index, the best extraction solvent was screened, and the soaking time of the material powder before extraction was determined.The optimum combination of material-liquid ratio, hydro-distillation time and powder granularity of the material were screened by single factor test combined with L9 (34) orthogonal test, the antibacterial activity was determined by the method of agar disc diffusion.The results showed that the optimum extraction conditions were as follows:ratio of material to distilled water was 1:20 g·mL-1, hydro-distillation time was 50 min, powder granularity of the material was 0.30～0.45 mm, the star anise powder was presoaked 36 h, petroleum ether (30～60 °C) as the extraction solvent and under these conditions the extraction rate could reach 14.657％.The inhibitory effect of star anise oil on Bacillus subtilis was better than that of Escherichia coli, and the antibacterial effect increased with the concentration of star anise oil.Though the antibacterial effect of oil phase of the volatile constituents of the star anise (e.g.star anise oil) was better than the water-soluble components, the water-soluble components with a concentration of 1 000.0 mg·mL-1 still showed moderate inhibition effect against E.coli and B.subtilis.Therefore, the volatile constituents of star anise (star anise oil and water-soluble components) should be used in combination.关键词
八角茴香油/水蒸气蒸馏法/提取工艺优化/抑菌活性Key words
star anise oil/hydro-distillation/optimization of extraction process/antibacterial activity分类
农业科技引用本文复制引用
李萍,汪青青,赵鹏英,周坤..水蒸气蒸馏法提取八角茴香油及其抑菌活性研究[J].天津农业科学,2019,25(2):7-11,5.基金项目
2017年天津农学院大学生创新创业训练计划项目(201710061210) （201710061210）
