中国组织工程研究2019，Vol.23Issue(9)：1319-1323,5.DOI:10.3969/j.issn.2095-4344.1642
CXCL12和CXCR4对骨髓干细胞向子宫内膜间质细胞趋化的影响
Effects of CXCL12 and CXCR4 on chemotaxis of bone marrow stem cells towards endometrial stromal cells
摘要
Abstract
BACKGROUND: Bone marrow stem cells can be colonized into the mouse endometrium. However, the molecular mechanisms underlying the migration of bone marrow stem cells into the endometrium are still unclear. OBJECTIVE: To investigate the effects of CXCL12 and CXCR4 on the chemotaxis of bone marrow stem cells towards endometrial stromal cells. METHODS: Mouse bone marrow stem cells and human endometrial stromal cells were serum-starved overnight. After 24 hours of estradiol pretreatment, the mRNA expressions of CXCR4 and CXCL12 in the mouse bone marrow stem cells were detected by RT-PCR; the levels of CXCL12 in the mouse bone marrow stem cells and human endometrial stromal cells were detected by ELISA; and the migration of mouse bone marrow stem cells into human endometrial stromal cells was observed by Transwell chamber test. RESULTS AND CONCLUSION: Estradiol pretreatment could upregulate the expression of CXCL12 in the human endometrial stromal cells as well as the expression of CXCR4 in the mouse bone marrow cells, promoting the migration of mouse bone marrow stem cells towards the conditioned medium of human endometrial stromal cells. In conclusion, estradiol can promote the migration of bone marrow stem cells into the endometrium, which may provide new ideas for the treatment of endometrial diseases.关键词
骨髓干细胞/子宫内膜间质细胞/CXCL12/CXCR4/雌二醇分类
医药卫生引用本文复制引用
黄晓萍,张娟,王文平,范波..CXCL12和CXCR4对骨髓干细胞向子宫内膜间质细胞趋化的影响[J].中国组织工程研究,2019,23(9):1319-1323,5.基金项目
四川省医学科研课题计划(S15026),项目负责人:黄晓萍 （S15026）
