中国组织工程研究2019，Vol.23Issue(10)：1526-1532,7.DOI:10.3969/j.issn.2095-4344.1596
脱细胞鱼皮口腔引导组织修复膜的制备和评价
Preparation and evaluation of fish skin acellular dermal matrix for oral guided tissue regeneration
王园园 1宋文山 1于德君 1代元坤 1李八方2
作者信息
- 1. 青岛海洋生物医药研究院,山东省青岛市 266100
- 2. 中国海洋大学食品科学与工程学院,山东省青岛市 266003
- 折叠
摘要
Abstract
BACKGROUND: Current oral restoration membranes are expensive with a potential risk of zoonotic virus, so new membranes are in need. OBJECTIVE: To prepare fish skin acellular dermal matrix and evaluate its efficiency of decellularization, biomechanical properties and biocompatibility, in order to explore its feasibility as an oral restoration membrane. METHODS: The skin of Astroconger Myriaster was harvested to prepare acellular dermal matrix by decellularization via a combination of physical method and chemical and biological reagents. The micromorphology of the material was observed under scanning electron microscope. Tissue composition was analyzed by hematoxylin-eosin, Masson and PAS staining. The DNA content was detected using a kit. The biocompatibility was evaluated through cytotoxicity and animal skin stimulation tests. RESULTS AND CONCLUSION: The prepared acellular dermal matrix: (1) was white or light yellow, with strength and toughness; (2) exhibited a double-layered structure composed of a dense layer and a loose layer; (3) was mostly composed of collagen without PAS positive substance, showing good decellularization; (4) had a significantly lower DNA content than natural fish skin and commercial oral restoration membrane; and (5) possessed good biocompatibility, with very low cytotoxicity and no stimulation to the animal skin. Therefore, the prepared acellular dermal matrix of fish skin shows a potential in the preparation of oral guided tissue regeneration membrane.关键词
口腔修复膜/脱细胞/真皮基质/鱼皮/引导组织修复/细胞毒性/生物相容性/力学性能/口腔材料/组织构建分类
医药卫生引用本文复制引用
王园园,宋文山,于德君,代元坤,李八方..脱细胞鱼皮口腔引导组织修复膜的制备和评价[J].中国组织工程研究,2019,23(10):1526-1532,7.
