中国组织工程研究2019，Vol.23Issue(10)：1546-1551,6.DOI:10.3969/j.issn.2095-4344.1626
国产多孔钽对MG63细胞中Ⅰ型胶原、谷氨酰转氨酶2和钙结合蛋白A4表达的影响
Effects of domestic porous tantalum on expression of collagen type I, tissue transglutaminase 2 and calcium-binding protein A4 in MG63 cells
摘要
Abstract
BACKGROUND: At present, there is evidence that domestic porous tantalum has good biocompatibility and osteogenic properties, but the specific osteogenic mechanism and its effect on osteogenic factors are still unclear. OBJECTIVE: To observe the effects of domestic porous tantalum materials on the expression of collagen type I, tissue transglutaminase-2 and calcium-binding protein A4 in MG63 cells. METHODS: MG63 cells in logarithmic growth phase were inoculated onto 24-well plates and cultured in three groups: in blank group, conventional medium was added; in tantalum extract group, porous tantalum material extract was added; and in tantalum scaffold group, porous tantalum material and conventional medium were added. On 1, 3, 5, 7 and 9 days of culture, the cell proliferation of each group was detected by cell counting kit-8 method. On 5 days of culture, the levels of collagen type I, tissue transglutaminase-2 and calcium-binding protein A4 secreted by MG63 cells in each group were detected by ELISA. Western blot assay was used to detect the expression of three proteins in each group. RESULTS AND CONCLUSION: (1) With the prolongation of culture time, the number of cells in each group increased gradually. There was no difference in cell proliferation among the three groups at different time points (P> 0.05). (2) The secretory levels of collagen type I and tissue transglutaminase-2 in the tantalum scaffold group were significantly higher than those in the blank group and tantalum extract group (P < 0.05), while the secretion of collagen type I and tissue transglutaminase-2 in the tantalum extract group was significantly higher than that in the blank group (P < 0.05). The secretion of calcium-binding protein A4 in the tantalum scaffold group was significantly lower than that in the other two groups (P < 0.05). (3) The expression of collagen type I and tissue transglutaminase-2 protein in the tantalum scaffold group was significantly higher than that in the blank group and tantalum extract group (P < 0.05), while the expression of collagen type I and tissue transglutaminase-2 protein in the tantalum extract group was significantly higher than that in the blank group (P < 0.05). The expression of calcium-binding protein A4 in the tantalum scaffold group was significantly lower than that in the blank group and tantalum extract group (P < 0.05). To conclude, domestic porous tantalum materials could promote the secretion of collagen type I and tissue transglutaminase-2 by MG63 cells, and inhibit the secretion of calcium-binding protein A4.关键词
国产多孔钽/MG63细胞/Ⅰ型胶原/谷氨酰转氨酶2/钙结合蛋白A4/钽浸提液分类
医药卫生引用本文复制引用
陈婧婧,王茜,崔逸爽,滕雪峰,张辉,李琪佳..国产多孔钽对MG63细胞中Ⅰ型胶原、谷氨酰转氨酶2和钙结合蛋白A4表达的影响[J].中国组织工程研究,2019,23(10):1546-1551,6.基金项目
国家科技部科技支撑课题资助项目(2012BAE06B03) （2012BAE06B03）
河北省科技支撑资助项目(16277776D),项目负责人:李琪佳 （16277776D）
河北省医学科学研究重点课题计划资助项目(20160225) （20160225）
华北理工大学博士科研启动基金资助项目(28606299),项目负责人:王茜 （28606299）
河北省卫计委资助课题(20180733),项目负责人:王茜 （20180733）
