中国组织工程研究2019，Vol.23Issue(14)：2196-2201,6.DOI:10.3969/j.issn.2095-4344.1664
可降解锌合金植入材料的体内抗菌性
Antibacterial properties of biodegradable zinc alloys in vivo
谢中 1羊明智 1薛静波 1陈勇 1彭立军 1蔡斌 1张杨洋1
作者信息
- 1. 南华大学附属第一医院脊柱外科,湖南省衡阳市 421001
- 折叠
摘要
Abstract
BACKGROUND: Zinc, an inorganic antibacterial material, has a suitable degradation rate and good antibacterial property. Adding alloying elements can improve the mechanical properties and biocompatibility of the material, which is the development direction of novel medical biodegradable metal materials. There is still lack a comparable research on the antibacterial properties among zinc-based materials. OBJECTIVE: To investigate the antibacterial properties of pure zine and zinc-based alloys in vivo. METHODS: Eighty Sprague-Dawley rats, SPF grade, were randomized into two groups (n=40/group) , and all rats were injected with Staphylococcus aureus or Escherichia coli solution to prepare infection models. Different materials (Zn, ZnAl, ZnSr, Zn3 Mg, Zn3 Ag, Zn3 Ca and Zn4 Cu; five rats for each material) were implanted into the medullary cavity of femur. The control group without any material was set. At 1, 4, 7 and 14 days after implantation, the changes of body temperature, white blood cell count, serum tumor necrosis factor α and serum zinc content in rats were detected. The secretions and tissues of the surgical site were collected to identify the bacterial species. RESULTS AND CONCLUSION: (1) The body temperature in all the rats was increased to different extents after bacterial infection, but the temperature of the rats implanted with zinc and zinc alloys was always lower than that in the control group at 7 and 14 days (P < 0.05) . The temperature in the Zn3 Ag group was significantly lower than that in the other groups at 7 and 14 days (P < 0.05) . (2) The white blood cell count and tumor necrosis factor α level in the zinc and zinc alloys groups were significantly lower than those in the control group at 7 and 14 days after implantation (P < 0.05) . The white blood cell count and tumor necrosis factor α level in the Zn3 Ag group were significantly lower than those in the other groups (P < 0.05) . (3) The serum zinc content in all groups has no significant difference (P> 0.05) . (4) The bacterial culture results showed S.aureus (+) in the Staphylococcus aureus infection group and E.coli (+) in the Escherichia coli infection group. (5) To conclude, degradable zinc-based alloys exert marked antibacterial effects, and Zn3 Ag alloys have the best antibacterial activity.关键词
可降解材料/锌合金/金黄色葡萄球菌/大肠杆菌/植入物/抗菌性/骨髓感染/动物实验Key words
Zinc/Prostheses and Implants/Tissue Engineering分类
医药卫生引用本文复制引用
谢中,羊明智,薛静波,陈勇,彭立军,蔡斌,张杨洋..可降解锌合金植入材料的体内抗菌性[J].中国组织工程研究,2019,23(14):2196-2201,6.
