新疆农业科学2018，Vol.55Issue(10)：1765-1774,10.DOI:10.6048/j.issn.1001-4330.2018.10.001
甜瓜果长基因fl与性别表达基因a的遗传分析及定位
Genetic Analysis and Primary Localization of Fruit Length Gene fl and Sex Expression gene a in Melon (Cucumis melo L.)
摘要
Abstract
[Objective] To identify the linkage relationship between fruit length gene fland sex expression gene a on LG2 (Linkage Group 2) in melon, 160 BC1 plants derived from the cross between the andromonoecious melon line 'Xizhoumi' with round fruit and the monoecious melon line 'snake melon' with oblong fruit were used to investigate the genetic mechanism and perform the primary gene localization for fruit length and sex expression type (andromonecious, monecious).[Method]Basing on the phenotypic frequency distribution of the above two traits, the genetic mechanism was analyzed. By BSA (Bulked Segregant Analysis), 48 SSR markers on LG2 were screened to obtain the polymorphism SSR markers for the two traits. And the hermaphroditic genes were primarily localized.[Result]The result of genetic analysis showed that the fruit length conformed to the QTL genetic mechanism, and sex expression type (andromonecious, monecious) was possibly controlled by the two genes. The result of linkage analysis showed that fruit length gene fl was localized between marker SSR247159 and marker SSR252089 on LG2, the genetic distance was 3 cm; sex expression gene a was localized between marker SSR227156 and marker CMGA36/SSR235092 on LG2, the genetic distance was 3.59 cm; the genetic distance between the two intervals was 0.6 cm. [Conclusion]In the BC1 populations derived from the cross between 'Xizhoumi' and 'snake melon', the fruit length gene fl and the sex expression gene a were localized in different marker regions, which proved that the two genes were not the same gene.关键词
甜瓜/果长基因/性别表达基因a/遗传定位Key words
muskmelon/fruit length gene/sex expression gene a/genetic localization引用本文复制引用
范文林,王贤磊,李群,俞志杰,李冠..甜瓜果长基因fl与性别表达基因a的遗传分析及定位[J].新疆农业科学,2018,55(10):1765-1774,10.基金项目
新疆维吾尔自治区自然科学基金项目 (2016D01C066) （2016D01C066）
