新疆农业科学2018，Vol.55Issue(10)：1927-1935,9.DOI:10.6048/j.issn.1001-4330.2018.10.020
不同油气储藏区微生物代谢活性及多样性响应
Microbial Metabolic Activity and Diversity Response in Different Oil and Gas Storage Areas
摘要
Abstract
[Objective] To study the response of soil microbial community metabolic activity and diversity to underground oil and gas storage.[Method]The metabolic activity and diversity of microbial community in 80-100 cm soil samples from different oil and gas storage areas in Yanqi were studied by using Biolog-ECO microplate technique.[Result]In the three areas of Yanqi, Xinjiang that the study analyzed, soil microbial communities were obviously affected by oil and gas distribution. The general rules of microbial metabolic activity were as follows: the microbial activity of favorable reservoir oil and gas region＞ unfavorable or difficult to evaluate reservoir oil and gas area＞ no reservoir oil and gas area; The contribution of amino acid and polymer utilization microbes to AWCD in the samples that were favorable to reservoir oil and gas or difficult to evaluate was higher than that in the control area, while carboxyl and amines were lower than those in the control area. Results of principal component analysis showed that the distribution characteristics of microbial communities in three different reservoirs were obviously different, and the most powerful matrixes was the D-mannitol and D-xylose in PCA 1 and PCA 2, whose respective load values were 0.97 and 0.87.[Conclusion]Biolog-ECO microplate technology can be used as a means for preliminary exploration of oil and gas reservoirs.关键词
Biolog-ECO/油气储藏/微生物代谢多样性/群落代谢活性Key words
biolog-ECO/oil and gas storage/microbial metabolic diversity/metabolic activity of community引用本文复制引用
楚敏,李玉国,欧提库尔,张涛,林青,张志东,高雁,史应武,杨红梅,霍向东,顾美英,曾军..不同油气储藏区微生物代谢活性及多样性响应[J].新疆农业科学,2018,55(10):1927-1935,9.基金项目
新疆维吾尔自治区自然科学基金"油气微生物勘探技术中微生物异常检测方法创新研究" (31460027) （31460027）
