烟草科技2019，Vol.52Issue(5)：6-13,8.
枯草芽孢杆菌BC80-6发酵条件的优化及对烟草根黑腐病的控病效果
Optimization of fermentation conditions for Bacillus subtilis BC80-6 and its control on Thielaviopsis basicola of tobacco
摘要
Abstract
To clarify the effects of Bacillus subtilis BC80-6 on Thielaviopsis basicola, the inhibitory effects of three treatment solutions of B. subtilis BC80-6 on the mycelial growth and spore germination of T. basicola were investigated. BC80-6 fermentation conditions were optimized and field control experiments were carried out. The results showed that 10-1 times living bacteria solution of B. subtilis BC80-6 had the best inhibitory effects on the mycelial growth and spore germination of B. subtilis with the inhibition rates of 93.2% and 95.3% , respectively. Via a single factor test and an orthogonal experiment, the optimum BC80-6 medium was 1.5% sucrose, 0.5% yeast extract and 0.06% magnesium sulfate (mass/volume ratio). The best fermentation condition was fermentation temperature 28 ℃, time 24 h, initial pH 6.5, rotation speed 180 r/min, inoculation amount 5% (v/v) and medium volume 40 mL in 250 mL triangular bottle. The field control effect of living bacteria solution of B. subtilis on tobacco root black rot was 88.19%.关键词
烟草/根黑腐病菌/枯草芽孢杆菌/发酵条件/田间防效Key words
Tobacco/Thielaviopsis basicola/Bacillus subtilis/Fermentation condition/Field control effect分类
农业科技引用本文复制引用
王文凤,张丽娜,朱启法,汪文杰,周本国,檀根甲,许大凤..枯草芽孢杆菌BC80-6发酵条件的优化及对烟草根黑腐病的控病效果[J].烟草科技,2019,52(5):6-13,8.基金项目
广西中烟工业有限责任公司2018年技术创新项目"卷烟爆珠中重点化学成分检测分析方法及产品质量评价研究"(GXZYCX2018C002). （GXZYCX2018C002）
