医学信息2019，Vol.32Issue(4)：5-7,3.DOI:10.3969/j.issn.1006-1959.2019.04.002
去睾丸老年大鼠血管钙化程度与骨代谢水平研究
Study on the Degree of Vascular Calcification and Bone Metabolism in Aged Test Rats
摘要
Abstract
Objective To observevascular calcification, bone mineral density (BMD) and bone metabolism markers in testicular aged rats [type Ⅰ procollagen N-terminal propeptide (P ⅠNP) , osteocalcin (BGP) and type Ⅰ collagen cross-linked carboxy terminal peptide (CTX) -Ⅰ) ] changes.Methods 16 male Sprague-Dawley rats aged 7 months were randomly divided into two groups. In the model group, the rat osteoporosis model was established by bilateral testicular extraction, and the control group was given sham operation. Six months after operation, all rats were sacrificed. BMD was measured by dual-energy X-ray absorptiometry. The thoracic aorta was stained with alizarin red to observe calcification. Serum P1 NP and BGP were measured by enzyme-linked immunosorbent assay and CTX-Ⅰ concentration. Results The BMD of the model group was lower than that of the control group [ (0.2636±0.0096) g/cm2 vs (0.3104±0.0157) g/cm2], the difference was statistically significant (P<0.05); The calcification of the thoracic aorta was obvious, and the serum PⅠNP and BGP levels were significantly lower than those of the control group (P<0.05) , while the serum CTX-Ⅰwas higher than the control group, the difference was statistically significant (P<0.05). Conclusion Osteoporosis model can be established in the aged rats by castration method, and the degree of vascular calcification is significantly increased during the process of osteoporosis formation, bone formation markers are decreased, and bone resorption markers are increased.关键词
去睾丸大鼠/老年性骨质疏松/血管钙化/骨代谢Key words
Testicular rats/Senile osteoporosis/Vascular calcification/Bone metabolism分类
医药卫生引用本文复制引用
杨娜,周进平,林晓容,徐军霞..去睾丸老年大鼠血管钙化程度与骨代谢水平研究[J].医学信息,2019,32(4):5-7,3.基金项目
福建省自然科学基金(编号:2017J01315) （编号:2017J01315）
