中风与神经疾病杂志2019，Vol.36Issue(1)：3-6,4.
Akt对IL-1β诱导神经干细胞分化为多巴胺能神经元的作用
Effect of Akt in the differentiation of dopaminergic neurons induced by IL-1β form the neural stem cells
摘要
Abstract
Objective To investigate the effect of Protein kinase B/Akt signal pathway in the process of neural stem cells on the differentiation of dopaminergic neurons induced by IL-1β. Methods The rat neural stem cells form the middle brain were obtained and cultured primitively and the differentiation was induced by IL-1β. Immunofluorescene staining method was used to detect the expression of beta III-tubulin and tyrosine hydroxylase (TH); Motic Digital Class 1. 1/Motic Images Advanced 3. 1 image analysis software was used to measure neurite's length of the dopaminergic neurons; Western blot was used to analyze the degree of Akt phosphorylation of differentiated cells induced by IL-1β. Results The cultured cells expressed nestin protein and differentiated into neurons and glial cells; The differentiation rate of beta III-tubulin positive cells was 13. 4％ ± 0. 8％ (group A) , 16. 8％ ± 1. 5％ (group B) , 28. 8％ ± 2. 4％ (group C) , 29. 2％ ± 1. 5％ (group D) , 12. 5％ ± 0. 6％ (control group) , the percentage of beta III-tubulin positive cells increased with the increase of IL-1βdose (P < 0. 05); The positive rate of TH positive cells was 1. 6％ ± 0. 5％ (group A) , 6. 0％ ± 1. 6％ (group B) , 24. 8％ ±1. 5％ (group C) 、24. 6％ ± 1. 4％ (group D) 、1. 5％ ± 0. 5％ (control group) , TH positive differentiated cells increased with the increase of IL-1β dose (P < 0. 01); The length of dopaminergic neurons' Bneurites was (48. 6 ± 8. 3) μm (group A) , (56. 4 ± 5. 2) μm (group B) , (80. 0 ± 12. 5) μm (group C) , (80. 2 ± 10. 5) μm (group D) , (45. 5 ± 6. 5) μm (control group) , the neurites' length of dopaminergic neurons increased with the increase of IL-1β dose (P < 0. 05) , and the level of phosphorylation of Akt in differentiated cells increased after IL-1 beta (P < 0. 01) . Conclusion IL-1β induced neural stem cells differentiate into dopaminergic neurons as a type of crucial signal molecules, the phosphorylation level of Akt increases during the differentiation, and Protein kinase B/Akt signal pathway may be activated by phosphorylation and involved in the regulation of neural stem cells to dopaminergic neurons.关键词
神经干细胞/Akt/IL-1β/多巴胺能神经元/诱导Key words
Neural stem cells/Akt/IL-1 bate/Dopaminergic neurons/Induction分类
医药卫生引用本文复制引用
种堔,董美希,刘莹,范国兵,张敏娜,王光辉,吴景龙..Akt对IL-1β诱导神经干细胞分化为多巴胺能神经元的作用[J].中风与神经疾病杂志,2019,36(1):3-6,4.基金项目
国家自然科学基金项目(61473043) （61473043）
国家教育部大学生创新创业基金项目(201610443071) （201610443071）
