中风与神经疾病杂志2019，Vol.36Issue(1)：10-14,5.
α-突触核蛋白通过抑制 Wnt /β-catenin信号通路参与帕金森病发病机制
α-synuclein Participate in the pathogenesis of Parkinson' s disease by inhibiting Wnt/β-catenin signaling
摘要
Abstract
Objective To investigate the effect of α-synuclein(α-syn) on Wnt /β-catenin signaling pathway in Parkinson' s disease animal models and cell models and the possible mechanism of neuronal injury. Methods Western blotting,flow cytometry,immunofluorescence and other methods were used to detect the expression of α-synuclein and the key signal molecule GSK-3β in Wnt /β-catenin signaling pathway and its effect on cell viability at the animal and cell levels. Results The expressions of α-synuclein and p-GSK-3β in the brain tissue of α-syn transgenic mice increased significantly,compared with the control group and MPTP group(P < 0. 05) . The expression levels of α-synuclein and p-GSK-3β in SH-SY5Y cells of α-syn overexpression group were significantly higher than those in the control group and MPP + injury group,and the results were statistically significant(P < 0. 05) . The α-synuclein in the aripiprazole pretreatment group was significantly increased compared with the control group and the MPP + group. There was no significant difference in pGSK-3β between the control group and the MPP + group. The cell viability of aripiprazole pretreatment group was significantly higher than that of α-syn overexpression group,and the apoptosis rate was significantly lower than that of α-syn overexpression group,the difference was statistically significant(P < 0. 05) . Immunofluorescence showed a colocalization relationship between α-synuclein and p-GSK-3β. Conclusion α-synuclein may participate in the pathogenesis of Parkinson' s disease by inhibiting the Wnt /β-catenin signaling pathway leading to neuronal damage.关键词
帕金森病/α-突触核蛋白/Wnt /β-catenin 信号通路Key words
Parkinson' s disease/α-synuclein/Wnt /β-catenin signaling pathway分类
医药卫生引用本文复制引用
程丽萍,王 浩,车峰远,亓法英..α-突触核蛋白通过抑制 Wnt /β-catenin信号通路参与帕金森病发病机制[J].中风与神经疾病杂志,2019,36(1):10-14,5.基金项目
山东省自然科学基金(No.ZR2014HL041) （No.ZR2014HL041）
