中国肺癌杂志2019，Vol.22Issue(1)：6-14,9.DOI:10.3779/j.issn.1009-3419.2019.01.03
人源性的小细胞肺癌异种移植动物模型及耐药模型的建立
Establishment of A Patient-derived Xenotransplantation Animal Model for Small Cell Lung Cancer and Drug Resistance Model
摘要
Abstract
Background and objective Small cell lung cancer (SCLC) is characterized by poor differentiation, high malignancy and rapid growth fast, short double time, early and extensive metastatic malignancy.In clinical, chemotherapy is the main treatment method, while resistance to multiple chemotherapy drugs in six to nine months has been a major clinical challenge in SCLC treatment.Therefore, It has important clinical value to building SCLC aninimal model which is similar to patients with SCLC.Animal model of xenotransplantation (PDX) from the patients with small cell lung cancer can well retain the characteristics of primary tumor and is an ideal preclinical animal model.The study is aimed to establish SCLC PDX animal model and induce the chemoresistance model to help to study the mechanism of chemoresistance and individual treatment.Methods Fresh surgical excision or puncture specimens from SCLC patients were transplanted into B-NSGTM mice subcutaneous tissues with severe immunodeficiency in one hour after operation the B-NSGTM mice subcutaneous in 1 hour, and inject chemotherapy drugs intraperitoneally after its tumor growed to 400 mm3 with EP which is cisplatin 8 mg/kg eight days and etoposide 5 mg/kg every two days until 8 cycles.Measure the tumor volum and mice weights regularly, then re-engrafted the largest tumor and continue chemotherapy.Results Nine cases were conducted for B-NSG mice modeling.Three of nine cases could be engrafted to new B-NSG mice at least two generation.The SCLC PDX animal models have been established successfully.After adopting chemotherapy drugs, the chemoresistance PDX models have been established.High homogeneity was关键词
小细胞肺癌/耐药/人源性异种移植/动物模型/B-NSGTM鼠Key words
Small cell lung cancer/Drug resistance/Patient-derived xenotransplantation/Animal model/B-NSGTM mice引用本文复制引用
朱亚如,黄卫妹,吴源周,贾龙飞,李雅玲,陈瑞,郭琳琅,陈群清..人源性的小细胞肺癌异种移植动物模型及耐药模型的建立[J].中国肺癌杂志,2019,22(1):6-14,9.基金项目
国家自然科学基金(重点)(No.81572244) （重点）
广东省自然科学基金(重点)(No.2015A030311028) （重点）
南方医科大学临床研究创新项目(No.LC2016ZD029)资助 （No.LC2016ZD029）
