中国感染控制杂志2019，Vol.18Issue(2)：99-104,6.DOI:10.12138/j.issn.1671-9638.20193929
耐碳青霉烯类肠杆菌科细菌对喹诺酮类耐药机制的研究
Mechanisms of quinolone resistance of carbapenem-resistant Enterobacteriaceae
摘要
Abstract
Objective To study the prevalence of plasmid-mediated quinolone resistance (PMQR) genes in carbapenem-resistant Enterobacteriaceae (CRE) and its resistance mechanism.Methods Clinically isolated CRE strains in a hospital from March 2015 to March 2018 were collected, then identified and performed antimicrobial susceptibility test by VITEK2 Compact analyzer, carriage of PMQR genes qnrA, qnrB, qnrS, qepA and acc (6') Ib-cr were determined by polymerase chain reaction (PCR) and sequencing, the horizontal transfer of PMQR genes were verified by plasmid conjugation test.Results Resistance rates of carbapenem-resistant Escherichia coli and carbapenem-resistant Klebsiella pneumoniae to quinolones were 100％ and 15.56％-33.33％ respectively.Detection rate of acc (6') Ib-cr gene was the highest (87.72％), followed by qnrB (77.19％) and qnrS (17.54％), 2 strains (3.51％) carried qnrA gene, qepA gene was not isolated, 84.21％ of strains harbored 2 or 3 PMQR genes.PMQR gene was transfected into all the 8 conjugated strains, but minimum inhibitory concentration value of quinolones didn't change significantly.Conclusion The detection rate of PMQR genes in CRE in this hospital is high, but there is a certain sensitivity to quinolones.关键词
喹诺酮耐药基因/肠杆菌科细菌/耐碳青霉烯类肠杆菌/耐药性Key words
quinolone resistance gene/Enterobacteriaceae/carbapenem-resistant Enterobacteriaceae/drug resistance分类
医药卫生引用本文复制引用
苏珊珊,张吉生,王英,王勇,王宇超,李慧玲,张晓丽..耐碳青霉烯类肠杆菌科细菌对喹诺酮类耐药机制的研究[J].中国感染控制杂志,2019,18(2):99-104,6.基金项目
黑龙江省自然科学基金(D201224) （D201224）
黑龙江省卫生计生委科研项目(2017-405) （2017-405）
佳木斯大学学科交叉项目(12J201505) （12J201505）
