局解手术学杂志2019，Vol.28Issue(1)：10-15,6.DOI:10.11659/jjssx.05E018039
miRNA-10b靶向调控Twist促进骨肉瘤细胞增殖与侵袭的研究
MicroRNA-10b promotes the proliferation and invasion of osteosarcoma cell lines by targeting twist gene
梁秀伟 1孙超 2文坤树 1蔡勇平 1高劲松 1李晓江1
作者信息
- 1. 重庆市梁平区人民医院骨科二病区,重庆 梁平 405200
- 2. 陆军军医大学第二附属医院骨科,重庆 400037
- 折叠
摘要
Abstract
Objective To investigate the role of microRNA-10b (miR-10b) in the proliferation and invasion potential of osteosarcoma cell lines MG-63 and the exact underlying mechanism.Methods The expression level of miR-10b in human osteosarcoma tissue samples and adjacent normal bone tissues were detected by relative quantitative real-time PCR (qRT-PCR).miR-10b mimic and siRNA against Twist (Twist siRNA) were transfected into human osteosarcoma cell lines MG-63 respectively using lipofactamine 2000, and RT-PCR was used to detect the mRNA expression levels of miR-10b and Twist, and Twist protein expression level was detected by Western blot.The effect of miR-10b mimic and Twist siRNA on proliferation of MG-63 were detected by MTT[3- (4, 5-dimethylthiazol-2-yl) -5- (3-carboxymethoxyphenyl) -2- (4-sulfophenyl) -2 H-tetrazolium].The in-vitro cell invasion ability was determined by Transwell invasion assays after up-regulating miR-10b or knocking down of Twist.Results The expression levels of miR-10b was higher in human osteosarcoma tissue samples compared with adjacent normal bone tissues, the differences were extremely statistical significance (P＜0.01).miR-10b directly up regulated the mRNA and protein expression levels of Twist, the differences were significant (P＜0.05).In addition, miR-10b had enhanced the cell invasion and the proliferation (P＜0.05), whereas the proliferation and invasion ability of MG-63 which transfected by both miR-10b mimic and Twist siRNA were significantly reduced than that transfected by miR-10b mimic (P＜0.05).Conclusion miR-10b in MG-63 promotes the proliferation and invasion potential of human osteosarcoma cell lines MG-63, at least partly through the upregulation of Twist gene.关键词
骨肉瘤/微小RNA-10b/Twist/细胞增殖/侵袭/细胞培养/细胞转染Key words
osteosarcoma/micro RNA-10b/Twist/cell proliferation/invasion/cell culture/cell transfection分类
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梁秀伟,孙超,文坤树,蔡勇平,高劲松,李晓江..miRNA-10b靶向调控Twist促进骨肉瘤细胞增殖与侵袭的研究[J].局解手术学杂志,2019,28(1):10-15,6.
