中国临床药理学杂志2019，Vol.35Issue(7)：640-642,3.DOI:10.13699/j.cnki.1001-6821.2019.07.012
瑞舒伐他汀对局灶性脑缺血再灌注大鼠炎性反应通路的影响
Effects of rosuvastatin on inflammatory response pathway of focal cerebral ischemia reperfusion in rats
摘要
Abstract
Objective To investigate the role of rosuvastatin in inhibiting inflammatory response pathway in rats with focal cerebral ischemia-reperfusion (I/R) . Methods Rats were randomly assigned into three groups: sham operation group, model group and experimental group, each group had 8 rats. The rats in the experimental group were lavaged with rosuvastatin 5 mg·kg-1·d-1 for 10 consecutive days before the model setting-up. The middle cerebral artery occlusion I/R model was made by the modified Zea-Longa wire bolt method. At 24 h after reperfusion, the positive cells of Toll like receptor 4 (TLR4) and nuclear transcription factor kappa B p65 (NF-κB p65) were measured by immunohistochemistry; the levels of the TLR4, NF-κB p65 protein and the TLR4, NF-κB p65 mRNA were measured by Western blotting and reverse transcription PCR, respectively. Results The expressions of TLR4 positive cells in sham group, model group and experimental group were (12. 69 ± 2. 55) cells, (38. 05 ± 6. 81) cells and (27. 80 ± 5. 75) cells, respectively;the expressions of NF-κB p65 positive cells in the three groups were (14. 64 ± 2. 79) cells, (47. 27 ± 7. 40) cells and (34. 83 ± 5. 60) cells, respectively; the expressions of TLR4 protein (optical density, OD) in the three groups were0. 28 ± 0. 04, 0. 63 ± 0. 09, 0. 41 ± 0. 07; the expressions of NF-κB p65 protein (OD) in the three groups were0. 21 ± 0. 04, 0. 72 ± 0. 12, 0. 36 ± 0. 06. Comparison between sham operation group and model group showed the difference of the factors were statistically significant (all P < 0. 05) ; comparison between model group and experimental group displayed the difference of the factors were statistically significant (all P < 0. 05) . The trend of gene results is consistent with that of protein. Conclusion Rosuvastatin can inhibit TLR4-NF-κB signal transduction pathway and reduce several inflammatory reaction after focal cerebral I/R.关键词
瑞舒伐他汀/脑缺血再灌注/Toll样受体4/核转录因子-κB p65Key words
rosuvastatin/cerebral ischemia-reperfusion/Toll like receptor 4/nuclear transcription factor kappa B p65分类
医药卫生引用本文复制引用
王杰华,李国前,洪诸权,宫淑杰,许秀秀,陈淑增..瑞舒伐他汀对局灶性脑缺血再灌注大鼠炎性反应通路的影响[J].中国临床药理学杂志,2019,35(7):640-642,3.基金项目
福建省教育厅B类课题基金资助项目 (JB13303) （JB13303）
泉州市卫生计生科研课题基金资助项目 （）
