中国临床药理学杂志2019，Vol.35Issue(7)：651-653,3.DOI:10.13699/j.cnki.1001-6821.2019.07.015
冬凌草甲素对急性哮喘小鼠Th1细胞/Th2细胞平衡的影响
Effect of oridonin on Th1 cell/Th2 cell balance in acute asthmatic mice
摘要
Abstract
Objective To observe the effect of oridonin on the Th1/Th2 balance induced by ovalbumin (OVA) in a mice model of asthma.Methods Balbc female mice were randomly divided into 4 groups:normal group, model group, and experimental-low, experimental-high groups, each group had 10 mice. The acute asthma model was induced by OVA. After the model was successfully established, the experimental group was injected with oridonin 20, 40 mg · kg-1, qd for 14 d. The normal group and the model group were given normal saline. The airway hyperresponsiveness of mice was detected by airway hyperresponsiveness instrument. Serum immunoglobulin E (IgE) and interleukin-4 (IL-4) were detected by enzyme-linked adsorption assay. The percentage of Th1, Th2 cells in spleen were detected by flow cytometry. Results The airway hyperresponsiveness values when the concentration of methacholine was 100 μg·m L-1 in model, experimental-low, experimental-high groups were 2. 99 ± 0. 56, 2. 72 ± 0. 68 and 2. 58 ± 0. 43, respectively.The serum IgE levels (OD values) in the three groups were 1. 13 ± 0. 17, 0. 76 ± 0. 21 and 0. 59 ± 0. 19, respectively. The serum IL-4 levels in the three groups were (66. 3 ± 2. 1) , (56. 7 ± 2. 5) and (42. 7 ± 2. 5) pg·m L-1. The Th1/Th2 in spleen of the three groups were 0. 67 ± 0. 04, 0. 99 ± 0. 04 and 1. 47 ± 0. 06, respectively.Comparison between experimental-low, experimental-high groups with model group, the differences of the factors were significant (P < 0. 05, P < 0. 01) . Conclusion Oridonin can reduce the level of serum IgE and regulate the differentiation of Th1/Th2, improvingthe inflammatory response of acute asthma model.关键词
冬凌草甲素/哮喘/Th1细胞/Th2细胞/免疫调节Key words
oridonin/asthma/Th1 cell/Th2 cell/immunomodulation分类
医药卫生引用本文复制引用
陈叶,胡智立,贺守第,肖小军,赵欣,冯斌,许峰..冬凌草甲素对急性哮喘小鼠Th1细胞/Th2细胞平衡的影响[J].中国临床药理学杂志,2019,35(7):651-653,3.基金项目
广东省科技计划基金资助项目 (2017A020215129) （2017A020215129）
深圳市南山区科技基金资助项目 (2017016) （2017016）
