中国临床药理学杂志2019，Vol.35Issue(7)：657-660,4.DOI:10.13699/j.cnki.1001-6821.2019.07.017
红芪多糖对糖尿病周围神经病变ob/ob小鼠高迁移率族蛋白1-Toll样受体4信号通路的影响
Effect of Hedysarum polybotrys polysacchcaide on high mobility group box protein1-Toll-like receptor 4 signaling pathway in ob/ob mice with diabetic peripheral neuropathy
摘要
Abstract
Objective To observe the effect of Hedysarum polybotrys polysacchcaide (HPS) on the signaling pathway of high mobility group box protein 1-toll-like receptor 4 (HMGB1-TLR4) in nervous tissue in diabetes mice with diabetic peripheral neuropathy (DPN) . Methods A total of 50 ten-week-old male ob/ob mice and 10 non-transgenic male db/m normal mice of the same age were randomly divided into6 groups. Normal group and model group were given 5 m L·kg-1·d-1 distilled water; experimental-L, experimental-M, experimental-H groups were given 50, 100, 200 mg·kg-1·d-1 HPS; control group was given 30 mg·kg-1·d-1 thioctanate, continuous gavage intervention for8 week. The blood glucose in each group was detected. At the end of treatment, the motor nerve conduction velocity of sciatic nerve was measured by PowerLab. The Western blot, polymerase chain reaction (PCR) were used to measure the protein and mRNA expressions of HMGB1, TLR4, nuclear transcription factor-κ B (NF-κB) and interleukin-1β (IL-1β) in sciatic nerve tissue. Results Eight weeks after treatment, the expressions of HGMB1 protein in normal group, model group and experimental-M, experimental-H groups were 0. 18 ± 0. 03, 0. 84 ± 0. 04, 0. 57 ± 0. 04 and 0. 37 ± 0. 08, compared with normal group, the HMGB1 protein level in model group had statistical difference (P < 0. 01) ; compared with model group, the HMGB1 protein levels in experimental-M and experimental-H groups had statistical difference (P < 0. 01, P < 0. 05) ; the expressions of TLR4 protein in normal group, model group and experimental-M, experimental-H groups were 0. 22 ± 0. 01, 1. 05 ± 0. 05, 0. 91 ± 0. 04 and0. 38 ± 0. 07, compared with normal group, the TLR4 protein level in model group had statistical difference (P < 0. 01) ; compared with model group, the TLR4 protein levels in experimental-M and experimental-H groups were significantly different (P < 0. 01, P < 0. 05) . The expression trenf of mRNA is consistent with that of its corresponding protein. Conclusion HPS can reduce the development of nervous injure by controlling inflammatory reaction through the HMGB1-TLR4 signaling pathway.关键词
红芪多糖/糖尿病周围神经病变/炎症反应Key words
Hedysarum polybotrys polysacchcaide/peripheral diabetic neuropathy/inflammatory reaction分类
医药卫生引用本文复制引用
何流,金智生,张磊,吉福玲,韩卫强..红芪多糖对糖尿病周围神经病变ob/ob小鼠高迁移率族蛋白1-Toll样受体4信号通路的影响[J].中国临床药理学杂志,2019,35(7):657-660,4.基金项目
国家自然科学基金地区科学基金资助项目 (81660777) （81660777）
