中国临床药理学杂志2019，Vol.35Issue(7)：667-670,4.DOI:10.13699/j.cnki.1001-6821.2019.07.020
盐酸右美托咪定对脂多糖诱导的库普弗细胞炎性反应的影响
Effect of dexmedetomidine hydrochloride on inflammatory response induced by lipopolysaccharide in Kupffer cells
摘要
Abstract
Objective To study the effect of dexmedetomidine hydrochloride (Dex) on lipopolysaccharide (LPS) induced inflammatory response in Kupffer cells. Methods Healthy male SD rats were selected for isolation and culture of liver Kupffer cells. The cells were randomly divided into 6 groups: normal group, model group, experimental-L group, experimental-H group, combined group and control group. The normal group was cultured routinely, model group was cultured with 1μg·m L-1 LPS solution 3 h, experimental-L, experimental-H group were cultured respectively with 0. 01 μmol·L-1 or 1 μmol·L-1 Dex 24 h + 1 μg·m L-1 LPS solution 3 h, combined group was cultured with 1μmol·L-1 Dex + 100 μmol·L-1 yohimbine hydrochloride (α2 adrenergicreceptor antagonist) 24 h + 1 μg·m L-1 LPS 3 h, control group was cultured with 100 μmol·L-1 yohimbine hydrochloride 24 h. The survival rate of Kupffer cells was determined by MTT. The contents of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the supernatant of Kupffer cells were determined by enzyme linked immunosorbent assay. Results After disposal, the survival rates of Kupffer cells in normal group, model group, experimental-L group, experimental-H group, combined group and control group were respectively (100. 00 ± 0. 00) ％, (56. 35 ± 4. 21) ％, (64. 78 ± 4. 37) ％, (83. 84 ± 5. 16) ％, (57. 03 ± 4. 09) ％ and (54. 58 ± 3. 95) ％. The TNF-αin the 6 groups were respectively (1. 19 ± 0. 16) , (57. 36 ± 7. 23) , (48. 27 ± 5. 58) , (7. 64 ± 0. 92) , (56. 98 ± 7. 05) and (58. 03 ± 7. 34) ng ·m L-1; the IL-6 in the 6 groups were respectively (47. 38 ± 6. 05) , (289. 49 ± 27. 83) , (231. 17 ± 22. 58) , (85. 35 ± 8. 23) , (287. 96 ± 26. 45) and (291. 43 ± 28. 04) pg·m L-1. Comparison between model group and normal group, the differences of the factors were significant (all P < 0. 05) ; comparison between two dose experimental groups and model group, the differences of the factors were significant (all P < 0. 05) ; comparison between combined group and control group with two dose experimental groups, the differences of the factors were significant (all P < 0. 05) . Conclusion Dex can reduce the activity of LPS induced Kupffer cells in a dose-dependent manner, inhibit oxidative stress injury and inflammatory response induced by Kupffer cells, which may be related to the agitation ofα2 adrenalic receptor. Yohimbine has antagonistic effect on its therapeutic effect.关键词
盐酸右美托咪定/脂多糖/库普弗细胞/氧化应激/炎性反应Key words
dexmedetomidine hydrochloride/lipopolysaccharide/Kupffer cell/oxidative stress/inflammatory response分类
医药卫生引用本文复制引用
林慧,伍东焕,林冠文,欧阳碧山..盐酸右美托咪定对脂多糖诱导的库普弗细胞炎性反应的影响[J].中国临床药理学杂志,2019,35(7):667-670,4.基金项目
海南省医药卫生科研基金资助项目 (1601320212A2001) （1601320212A2001）
