中国临床药理学杂志2019，Vol.35Issue(8)：750-752,3.DOI:10.13699/j.cnki.1001-6821.2019.08.007
阿替普酶静脉溶栓治疗急性脑梗死大鼠的疗效及对脑组织炎性因子表达的影响
Effect of antiprase intravenous thrombolysis in the treatment of acute cerebral infarction rats and the influence on the expression of inflammatory factors in brain tissue
林芬 1张云峰2
作者信息
- 1. 浙江省舟山市普陀区人民医院 药剂科,浙江 舟山 316100
- 2. 浙江省人民医院 药学部门诊药房,杭州 310000
- 折叠
摘要
Abstract
Objective To explore the effect of antiprase (r-tPA) intravenous thrombolysis in the treatment of acute cerebral infarction rats and the influence on the expression of inflammatory factors in brain tissue. Methods Forty-two acute cerebral infarction rats were randomly assigned to model group (n = 21) and test group (n = 21) ,while 20 healthy rats were assigned to sham-operation group. The test group was treated with r-tPA (5 mg·kg-1) by caudal vein injection within 3 h after infarction,and model group and sham-operation group were treated with equal amount of 0. 9％ NaCl. The volume of cerebral infarction after infarction were detected by chloride three phenyltetrazole (TTC) staining,the expression of inflammatory factors in brain tissue were detected by enzyme-linked immunosorbent assay (ELISA). Results On Day 7 after infarction,the volume of cerebral infarction in the model group were (86. 94 ± 4. 08) ％ and (42. 12 ± 2. 89) ％,and the differences was significant (P < 0. 05). On Day 7 after infarction,there were significant diffe-rences among the sham-operation group,model group and the test group in tumor necrosis factor-α(TNF-α) [(0. 32 ± 0. 07) pg·mL-1 vs(1. 36 ± 0. 12) pg·mL-1 vs(0. 27 ±0. 10) pg·mL-1] and intercellular adhesion molecule-1 (ICAM-1) [(1. 39 ± 0. 11) pg ·mL-1 vs(28. 79 ±2. 12) pg·mL-1 vs (17. 32 ±1. 91) pg·mL-1](all P <0. 05). Conclusion r-tPA intravenous thrombolysis treatment can effectively reduce the volume of cerebral infarction and the expression of TNF-α,ICAM -1 in brain tissue of acute cerebral infarction rats.关键词
急性脑梗死/静脉溶栓/阿替普酶/炎性因子/肿瘤坏死因子-α/细胞间黏附因子-1Key words
acute cerebral infarction/intravenous thrombolysis/antiprase/inflammatory/tumor necrosis factor-α/intercellular adhesion molecule -1分类
医药卫生引用本文复制引用
林芬,张云峰..阿替普酶静脉溶栓治疗急性脑梗死大鼠的疗效及对脑组织炎性因子表达的影响[J].中国临床药理学杂志,2019,35(8):750-752,3.
