中国临床药理学杂志2019，Vol.35Issue(8)：756-758,3.DOI:10.13699/j.cnki.1001-6821.2019.08.009
硝普钠对实验性脑出血大鼠神经功能及蛋白酪氨酸激酶2/信号传导与转录激活因子3通路的影响
Effects of sodium nitroprussiate on neurological function and Janus family of tyrosine kinases/signal transduction and transcription activator 3 pathway in rats with experimental intracerebral hemorrhage
鲍欢 1郝俊杰 1杨玉梅 1许嗣漪 1张小梅 2陈丽芳2
作者信息
- 1. 上海市东方医院 神经内科,上海 200120
- 2. 上海市东方医院吉安医院 神经内科,江西 吉安 343000
- 折叠
摘要
Abstract
Objective To investigate the effects of sodium nitroprussiate on neurological function and Janus family of tyrosine kinases/signal transduction and transcription activator 3 (JAK2/STAT3) pathway in rats with experimental intracerebral hemorrhage. Methods Sixty rats were randomly divided into control group,model group and test group,20 rats in each group. The model in model group and test group were given collagenase and heparin mixture to establish experimental cerebral hemorrhage,while control group was given the same amount of 0. 9％ NaCl. The test group received intraperitoneal injection with sodium nitroprussiate(1 mg·kg-1) at 6,24,72 and 120 h after modeling,and the rats in model group and control group received intraperitoneal injection with the same amount of 0. 9％ NaCl. The brain water content of each group was measured by weighing method was calculated; TdT-mediated dUTP nick- end labeling (TUNEL) method was used to detect apoptosis of rat brain tissue,and Western blotting (WB) method was used to detect the expression of JAK2/STAT3 pathway-related protein in rat brain tissue. Results At 72 h after modeling,there were significant differences among the control group,model group and test group in the brain water content [(76. 05 ±2. 04) ％ vs (84. 91 ±2. 58) ％ vs (79. 68 ±3. 01) ％],the rate of apoptotic neurons [(7. 52 ± 1. 86) ％ vs (50. 23 ± 2. 43) ％ vs (26. 24 ± 2. 37) ％],the relative expression of p- JAK2 protein in the brain tissues [(0. 07 ± 0. 13) vs (1. 26 ± 0. 05) vs (0. 33 ± 0. 05) ]and the relative expression of p-STAT3 protein [(0. 20 ± 0. 03) vs (0. 38 ± 0. 06) vs (0. 26 ± 0. 09) ](all P < 0. 05). Conclusion Sodium nitroprussiate has protective effect on brain injury in experimental cerebral hemorrhage rats,which may be related to regulate JAK2/STAT3 pathway and reduce apoptosis of brain tissue.关键词
高血压/脑出血/硝普钠/神经功能/蛋白酪氨酸激酶2/信号传导与转录激活因子3通路Key words
hypertension/intracerebral hemorrhage/sodium nitroprussiate/neurological function/Janus family of tyrosine kinases/signal transduction and transcription activator 3 pathway分类
医药卫生引用本文复制引用
鲍欢,郝俊杰,杨玉梅,许嗣漪,张小梅,陈丽芳..硝普钠对实验性脑出血大鼠神经功能及蛋白酪氨酸激酶2/信号传导与转录激活因子3通路的影响[J].中国临床药理学杂志,2019,35(8):756-758,3.
