中国临床药理学杂志2019，Vol.35Issue(8)：762-764,3.DOI:10.13699/j.cnki.1001-6821.2019.08.011
丝胶对糖尿病大鼠血视网膜屏障损伤的改善作用
Ameliorating effect of sericin on the blood-retinal barrier breakdown in diabetic rats
摘要
Abstract
Objective To investigate the ameliorating effect of sericin on the blood-retinal barrier breakdown in diabetic rats. Methods The diabetic models were established by feeding high-fat and high-sugar diets and intraperitoneal injection of streptozotocin. Successful model rats were randomized into model group and experimental group with 12 rats for each group,and another 12 normal matched rats served as normal group. The sericin solution 2. 4 g·kg-1·d-1 was gavage in the experimental group for 35 d; the other two groups were given intragastric administration of 0. 9％ NaCl of equal volume for 35 d. Blood-retinal barrier permeability was detected by evans blue(EB) method. The expression level of Occludin protein in retinas were detected by Western blot. The content of tumor necrosis factor-α (TNF-α) in retinas was detected by enzyme- linked immunosorbent assay. Results The amount of EB leakage in normal group,model group and experimental group were respectively(12. 42 ± 1. 40) ,(29. 07 ± 3. 22) ,(19. 59 ± 2. 45) ng·mg-1; the relative expression level of Occludin protein in the 3 groups were respectively0. 91 ± 0. 14,0. 20 ± 0. 04 and 0. 66 ± 0. 08; the content of TNF-α in the 3 groups were respectively (113. 31 ± 12. 48) ,(212. 09 ± 23. 19) ,(156. 05 ± 12. 84) ng · L-1. Comparison between model group and normal group, the difference of the factors were significant(all P < 0. 05); comparison between experimental group and model group, the difference of the factors were significant(all P < 0. 05). Conclusion Sericin can improve the blood- retinal barrier breakdown in diabetic rats and the mechanism may be related to inhibition of inflammatory cytokines expression.关键词
丝胶/糖尿病视网膜病变/血视网膜屏障/炎症Key words
sericin/diabetic retinopathy/blood-retinal barrier/inflammation分类
医药卫生引用本文复制引用
董志军,陈志宏,于宏飞,陶相宜,杨帆,董微丽..丝胶对糖尿病大鼠血视网膜屏障损伤的改善作用[J].中国临床药理学杂志,2019,35(8):762-764,3.基金项目
国家自然科学基金资助项目(81441133) （81441133）
河北省自然科学基金资助项目(H2013406096) （H2013406096）
承德市科技计划基金资助项目(201606A056) （201606A056）
