中国临床药理学杂志2019，Vol.35Issue(8)：765-769,5.DOI:10.13699/j.cnki.1001-6821.2019.08.012
阿司匹林联合肿瘤坏死因子相关凋亡诱导因子诱导剂通过增强自噬促进宫颈癌细胞凋亡
Aspirin combined with tumor necrosis factor-related apoptosis-inducing ligand inducer increases apoptosis of cervical cancer cells through autophagy enhancement
摘要
Abstract
Objective To explore the effect of aspirin(ASP) combined with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) inducer TIC10 on apoptosis and autophagy of cervical cancer Siha and HeLa cells. Methods HPV16 positive squamous carcinoma Siha cells and HPV18 positive cervical adenocarcinoma HeLa cells were selected as research objects. Two cell lines were treated with ASP,TIC10 or two drugs combination. The effects of ASP and TIC10 on cell proliferation were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay,cell migration ability was detected by cell scratch assay,cell clone formation ability was detected by plate clone formation assay,and the levels of cleaved Caspase-3/8,LC3A/B,Beclin1 were detected by Western Blot. Results The MTT showed that the proliferations of Siha and HeLa cells were inhibited by APS in a concentration dependent manner (all P < 0. 01). After treated with TIC10 combined with ASP for 48 h,the cell proliferation inhibition rates of Siha and HeLa cells were higher than thosetreated with TIC10 alone (all P < 0. 01). After treated with TIC10 or ASP for 24 h, the migration rates of Siha and He- La cells in the experimental groups [ASP: (19. 61 ± 1. 17) ％,(23. 75 ± 0. 78) ％; TIC10: (16. 89 ± 1. 47) ％,(20. 59 ± 2. 01) ％]were lower than those of control group [(41. 18 ± 2. 01) ％,(40. 83 ± 3. 77) ％],all P < 0. 01. After two weeks of treatmentof ASP and TIC10,the CFE(colony-forming efficiency) rates of Siha and HeLa cells in the experimental groups [ASP: (24. 93 ± 2. 12) ％, (26. 47 ± 3. 30) ％, TIC10: (17. 33 ± 1. 50) ％,(19. 13 ± 4. 99) ％]were lower than those of control group [(69. 60 ± 3. 54) ％,(68. 40 ± 4. 20) ％],all P < 0. 01. Western blot assays showed that the expression of cleaved Caspase-3/8 and autophagy related protein LC3A/B,Beclin1 were higher than those of control group (all P < 0. 01). Conclusion ASP increases cervical cancer cells apoptosis with TRAIL inducer TIC10 through autophagy enhancement.关键词
阿司匹林/子宫颈癌/Siha细胞/HeLa细胞/肿瘤坏死因子相关凋亡诱导因子/细胞自噬Key words
aspirin/cervical cancer/Siha/HeLa/tumor necrosis factor-related apoptosis-inducing ligand/cell autophagy分类
医药卫生引用本文复制引用
金彦斌,姜李乐,张怡,张翔,王文华,崔金全..阿司匹林联合肿瘤坏死因子相关凋亡诱导因子诱导剂通过增强自噬促进宫颈癌细胞凋亡[J].中国临床药理学杂志,2019,35(8):765-769,5.基金项目
河南省自然科学基金面上项目(162300410301) （162300410301）
