摘要
Abstract
Objective To investigate the hippocampal neurons apoptosis induced by propofol in neonatal rats and its relationship with RhoA/ROCK2 signaling pathway. Methods Forty-six SD rats were randomly divided into control group and exp-L/-H group,which were injected intraperitoneally with 7. 5 mL · kg-1 of fat emulsion and 50,100 mg·kg-1 of propofol,respectively. Neurons of neonatal SD rats were isolated and cultured,neurons of control group (C group) were cultured normally,while neurons of propofol intervention (P-L/-H) group were given 6,12 μg·mL-1 of propofol,respectively. The apoptosis of primary neurons of SD rats was detected by TUNEL,and the expression of caspase-3 and RhoA/ROCK2 signaling pathway protein were detected by Western blot. Results In C group and P-L/-H group,apoptosis rates were (9. 54 ± 1. 13) ％,(10. 53 ± 1. 85) ％,(27. 63 ± 2. 54) ％,respectively,and the neuron apoptosis rate of the P-H group was significantly higher than those of the C group and P-L group (P < 0. 05). The expression of Caspase-3 protein in hippocampus of control group,exp-L/-H group were 0. 28 ± 0. 03,0. 32 ± 0. 05,0. 98 ± 0. 25,the expression of ROCK2 protein were 0. 25 ± 0. 05,0. 32 ± 0. 06,0. 87 ± 0. 08,the expression of ROCK2 protein were 0. 59 ± 0. 11,0. 63 ± 0. 20,1. 53 ± 0. 09,respectively,and the indicators of the exp-H group was significantly higher than those of the control group and exp-L group (P < 0. 05). Conclusion High-dose Propofol can induce apoptosis of hippocampal neurons in neonatal rats,and the activation of RhoA/ROCK2 signaling pathway is involved in this process.关键词
异丙酚/新生大鼠/神经元凋亡/RhoA/ROCK2信号通路Key words
propofol/neonatal rats/neuronal apoptosis/RhoA/ROCK2 signaling pathway分类
医药卫生
