中国临床药理学杂志2019，Vol.35Issue(8)：776-779,4.DOI:10.13699/j.cnki.1001-6821.2019.08.015
谷氨酰胺对大鼠肠缺血再灌注损伤的保护作用及相关机制
Protective effects of glutamine on intestinal ischemia reperfusion injury in rats and its mechanism
摘要
Abstract
Objective To investigate the effects of glutamine on intestinal ischemia reperfusion injury(IR) in rats and the related mechanisms. Methods According to the weight, rats were divided into five groups: control group,experimental-A group,experimental-B group,experimental- C group and experimental-D group. The A group and B group were preoperatively given glutamine (1. 5 mg·kg-1) through tail vein injection and intragastric administration,respectively. The control group was preoperatively administered 1 mL 0. 9％ sodium chloride was given for the rat each day by tail vein injection. The experimental-C group and experimental-D group were given glutamine(1. 5 mg·kg-1) through injection of superior mesenteric vessel and intestinal cavity,respectively,after open abdomen,once a day for 4 days. Meanwhile,the model of intestinal IR was established by clamping the superior mesenteric vessel. Intestinal tissues and blood samples were taken after ischemia for 50 minutes,reperfusion for 1 h and 12 h. The changes of tumor necrosis factor-alpha(TNF-α) content in blood samples were detected by enzyme-linked immunosorbent assay. RT-PCR were used to detect nuclear factor kappa-B(NF-κB) and peroxisome proliferator-activated receptor gamma(PPAR-γ) mRNA in intestinal specimens of rats with intestinal IR. Western-blot were used to detect NF-κB and PPAR-γ protein expression. Results After intestinal reperfusion for 12 h, serum TNF-α levels in control,experimental-A,experimental-B,experimental-C and experimental-D groups were respectively(419. 04 ± 10. 55) ,(311. 77 ± 9. 81) ,(224. 53 ± 3. 36) ,(318. 77 ± 15. 64) and (436. 20 ± 22. 50) ng·L-1; comparison between experimental-A group and experimental-B group with control group, the difference was statistically significant (all P < 0. 05); comparison between experimental-A group and experimental-B group with experimental- D group,the difference was statistically significant (all P < 0. 05). The expression levels of NF-κB mRNA in the five groups were 1,0. 51 ± 0. 03,0. 61 ± 0. 07,0. 62 ± 0. 09 and 0. 92 ± 0. 05,respectively; meanwhile,the expression levels of PPAR-γ mRNA in the five groups were 1,2. 91 ± 0. 22,2. 52 ± 0. 24,1. 48 ± 0. 12 and1. 39 ± 0. 13,respectively; comparison between experimental-A group with control group and experimental-D group,the difference was statistically significant (all P < 0. 05). The trend of protein results is consistent with that of gene. The trend of 1 h results is consistent with that of 12 h. Conclusion Glutamine can play a protective role to intestinal IR rats via a mechanism of reducing the activity of NF-κB and increasing the activity of PPAR-γ.关键词
谷氨酰胺/肠缺血再灌注损伤/肠组织病理形态/核因子-κB/过氧化物酶体增殖物激活受体γKey words
glutamine/intestinal ischemia-reperfusion injury/pathological morphology of intestinal tissue/nuclear factor kappa-B/peroxisome proliferator-activated receptor gamma分类
医药卫生引用本文复制引用
华进,戴起宝..谷氨酰胺对大鼠肠缺血再灌注损伤的保护作用及相关机制[J].中国临床药理学杂志,2019,35(8):776-779,4.基金项目
国家临床重点专科建设基金资助项目(普通外科2013-GJLCZD) （普通外科2013-GJLCZD）
福建省中青年教师教育科研基金资助项目(JAT170239) （JAT170239）
