中国临床药理学杂志2019，Vol.35Issue(8)：801-803,3.DOI:10.13699/j.cnki.1001-6821.2019.08.022
葛根素对子痫前期大鼠模型胎盘氧化应激及免疫状态的影响
Effects of puerarin on oxidative stress and immune status of placenta in rats with preeclampsia
阚长利 1李久民 2侯敬 1景向阳 1朱艳菊 1张金环 1郭艳巍 1陈雪荣3
作者信息
- 1. 承德医学院 附属医院 南院区 产科,河北 承德 067000
- 2. 承德县中医院 内科,河北 承德 067400
- 3. 昆明医科大学 附属延安医院 产科,昆明 650051
- 折叠
摘要
Abstract
Objective To investigate the effect of puerarin on oxidative stress and immune status in placenta of preeclampsia (PE) rats. Methods Forty-five pregnant female SD rats were randomly divided into control group,model group and test group,15 rats in each group. The rats in model group and test group were subcutaneously injected with 100 mg·kg-1 of L-arginine methyl ester (L-NAME) once daily from Day 13-21 of gestation,the rats in control group were injected with the same amount of saline; and the rats in test group were intraperitoneally injected with 80 mg·kg-1 of puerarin solution once daily from Day 17-21 of gestation,control group and model group were injected with the same amount of 0. 9％ NaCl. The caudal arterial pressure and 24 h urinary protein content were measured at Day 10,16 and 21 of gestation,the oxidative stress indexes in rat placenta was detected by thibabituric acid method and Xanthine oxidase method,the cellular immune function was detected by flow cytometry,and the X-linked inhibitor of apoptosis protein (XIAP) expressionin of rat placenta trophoblasts was detected by Western blot. Results On Day 21 of gestation,there were significant differences between the model group and the control/test group in caudal arterial pressure [(136. 25 ± 5. 48) mm- Hg vs (119. 25 ± 4. 21) mmHg or (123. 52 ± 6. 45) mmHg],24 h-urinary protein contents [((11. 83 ± 0. 12) mg vs 6. 42 ± 0. 08) mg or (8. 58 ± 0. 12) mg],the content of MDA in placenta tissue [(4. 75 ± 0. 08) mmol·mg-1 vs(10. 69 ± 0. 07) mmol·mg-1,(8. 37 ± 0. 08) mmol·mg-1 ],the content of SOD [(93. 26 ± 4. 29) U·mg-1 vs(168. 97 ± 3. 42) U·mg-1 or (112. 63 ± 3. 48) U·mg-1 ],the ratio of CD4 +/CD8 + [(2. 61 ± 0. 56) vs(1. 46 ± 0. 05) or (1. 48 ± 0. 12) ] and the relative expression of XIAP protein in trophoblast [(0. 89 ± 0. 05) vs(0. 36 ± 0. 09) or (0. 41 ± 0. 11) ](all P < 0. 05). Conclusion Puerarin can inhibit the oxidative stress reaction and enhance the cellular immune function in the placenta of PE rats,and reduce the expression of XIAP protein in the placenta tissue trophoblasts to reduce the apoptosis of placenta trophoblasts.关键词
子痫前期/葛根素/氧化应激/免疫功能/凋亡Key words
preeclampsia/puerarin/oxidative stress/immune function/apoptosis分类
医药卫生引用本文复制引用
阚长利,李久民,侯敬,景向阳,朱艳菊,张金环,郭艳巍,陈雪荣..葛根素对子痫前期大鼠模型胎盘氧化应激及免疫状态的影响[J].中国临床药理学杂志,2019,35(8):801-803,3.
