中国蔬菜Issue(1)：36-40,5.
重组酶聚合酶扩增技术在番茄黄化曲叶病毒检测中的应用
Application of Recombinase Polymerase Amplification Technology in Detecting TYLCV
摘要
Abstract
Tomato yellow leaf curl virus (TYLCV) is an etiology of Tomato yellow leaf curl disease (TYLCD), which will cause serious damages to tomato production.It is of important significance to exploit rapid and accurate detection method for controlling this disease.According to DNA-A gene sequence of TYLCV, this study designed special RPA primers and established a method named TYLCV-RPA, and analyzed the specificity and sensitivity of this method.The results indicated that TYLCV-RPA method could amplify specific band of 343 bp from sample tomatoes with positive TYLCV.The reaction condition was 37 ℃ for 40 min.This method needed short time period for amplification with low requirement for equipment.It had no cross reaction with the other viruses and had good specificity.Its sensitivity was 10 times of the PCR method.It is suitable for rapid detection of TYLCV.关键词
番茄黄化曲叶病毒/重组酶聚合酶扩增/等温扩增/检测方法Key words
Recombinase polymerase amplification/Isothermal amplification/Detection method引用本文复制引用
周莹,杨丽梅,刘梅,黄金宝..重组酶聚合酶扩增技术在番茄黄化曲叶病毒检测中的应用[J].中国蔬菜,2019,(1):36-40,5.基金项目
北京市农林科学院青年科研基金项目(QNJJ201705) （QNJJ201705）
北京市优秀人才培养资助项目(2016000020060G131) （2016000020060G131）
北京市农林科学院创新能力建设项目(KJCX20180705) （KJCX20180705）
北京市农林科学院科技创新团队项目(JNKYT201605) （JNKYT201605）
