中国蔬菜Issue(2)：34-41,8.
基于InDel标记的大白菜育种材料分子身份证构建
Construction of Molecular Identity Card for Chinese Cabbage Breeding Materials Based on InDel Markers
摘要
Abstract
Taking 105 Chinese cabbage [Brassica campestris L. ssp. pekinensis (Lour) Olsson] breeding lines as test material, this paper screened out 20 pairs distributed evenly in Chinese cabbage genome, conducted molecular marker detection on the codominant InDel primers only produced 2 alleles, and built molecular identity cards.The results showed that 16 out of 20 pairs of primers produced single band, respectively. While, 2 pairs of primers each from partial materials were found loss and duplicated.All primers were ordered according to chromosome number, starting from A01 to A10 and primers on the same chromosome were ordered according to their physical position from small to large.According to these order, values were assigned to tags and 105 molecular identity cards of Chinese cabbage material were built.The screened out 20 pairs of primers had universality and compatibility for the identity cards construction of the unknown new germplasm resources, indicating that InDel marker technology could be used as an effective means for Chinese cabbage identification and molecular ID construction.关键词
大白菜/育种材料/InDel标记/分子身份证Key words
Chinese cabbage/Breeding line/InDel marker/Molecular ID (identity card)引用本文复制引用
刘栓桃,张志刚,王荣花,王立华,李巧云,赵智中..基于InDel标记的大白菜育种材料分子身份证构建[J].中国蔬菜,2019,(2):34-41,8.基金项目
山东省重点研发计划项目(2017GNC10124) （2017GNC10124）
山东省农业重大应用技术创新项目(2018) （2018）
山东省泰山学者计划项目(2016-2020) （2016-2020）
山东省农业科学院创新工程项目(CXGC2016A11) （CXGC2016A11）
