中国生物防治学报2019，Vol.35Issue(2)：197-202,6.DOI:10.16409/j.cnki.2095-039x.2019.02.014
对葱黄寡毛跳甲高毒力Bt菌株的鉴定及效果评价
Identification and Evaluation of High Virulence Bt Strain against Luperomorpha suturalis Adults
摘要
Abstract
A strain DK49 of high insecticidal activity against Luperomorpha suturalis was screened. Homology of the strain DK49 was compared using gene sequences of gyr B and pho R in Gen Bank separately, which showed high homology (99％) to that of Bacillus thuringiensis. Phylogenetic trees indicated that the strain DK49 is a B. thuringiensis strain. Biolog phenotypic analysis showed that the strain DK49 could effectively absorb 14 carbon sources and was sensitive to 10 chemicals and insensitive to 11 chemicals. Laboratory evaluation of bioactivity determined its 3 d LC50 against L. suturalis adult to be 0.8161×107 cfu/mL. Field assays with 3 continuous sprays at 1×107 cfu/mL showed a control efficiency of 96.81％ against L. suturalis. Therefore the strain DK49 has a potential for field control of L. suturalis.关键词
葱黄寡毛跳甲/苏云金芽胞杆菌/分子鉴定/活性评价Key words
Luperomorpha suturalis/Bacillus thuringiensis/molecular identification/activity evaluation分类
农业科技引用本文复制引用
宋健,曹伟平,张晓,杜立新..对葱黄寡毛跳甲高毒力Bt菌株的鉴定及效果评价[J].中国生物防治学报,2019,35(2):197-202,6.基金项目
国家重点研发计划 (2017YFD0201205) （2017YFD0201205）
河北省自然科学基金 (C2018301023) （C2018301023）
河北省财政项目 (C19R1001) （C19R1001）
河北省农林科学院科学技术研究与发展计划 (2018120302) （2018120302）
