中国输血杂志2019，Vol.32Issue(2)：123-127,5.DOI:10.13303/j.cjbt.issn.1004-549x.2019.02.007
无偿献血人群中HCV/OBI共感染者的分子流行病学特征
Molecular epidemiological characteristics of HCV/OBI co-infected blood donors
摘要
Abstract
Objective To investigate the prevalence of occult hepatitis B infection (OBI) among blood donors with chronic hepatitis C virus (HCV) infection in China, and provide a scientific basis for further clarifying the mechanism of HCV/OBI. Methods HBV DNA and anti-HBc were detected in 933 samples of HCV infected volunteer blood donors in Guangzhou from January 2011 to July 2015 using PROCLEIX TIGRIS, Roche Cobas s 201 and Roche E601. The differences of gender, age, nationality and ALT between the HCV-monoinfected and HCV/OBI co-infected blood donors were statistically analyzed. HCV C region and HBV S region were amplified by nested polymerase chain reaction (PCR). The evolutionary tree was constructed for genotyping, and the viral loads of HCV and HBV were detected by real time polymerase chain reaction (RT-PCR). In addition, 44 local HCV-monoinfected patients with HCV 6a genotype and 16 local HCV/HBV co-infected patients were randomly selected as the control group. The HCV C region of each group was amplified by nested PCR, and the amino acid sequences of HCV core protein in the three groups was compared and analyzed.Results Among 933 HCV-infected samples, 6 were HBV DNA and anti-HBc positive, and the OBI infection rate was 6.43 (6/933) among HCV-infected blood donors. Among 6 HCV/OBI co-infected individuals, HCV genotype 6a accounted for 83.3％ (5/6) and 1b accounted for 16.7％ (1/6). HCV viral load (log10 IU/mL) ranged from 3.8 to 5.7, with the mean value of 5.0±0.69. There was no significant differences in gender, age, nationality, native place and ALT between HCV-monoinfected group and HCV/OBI co-infected group (P>0.05). The amino acid variability of HCV core protein sequence in HCV-monoinfected group, HCV/HBV co-infected group and HCV/OBI co-infected group were 0.8±1.3, 0.7 ±1.5 and 0.4±0.5, respectively (P>0.05). No consistent structure or mutation pattern was found in HCV/OBI co-infection group.Conclusion There is a higher positive rate of OBI among the volunteer blood donors in Guangzhou, and the proportion of HCV 6 a genotype in the co-infection of HCV/OBI was significantly higher than that of type 1b.关键词
丙型肝炎病毒/乙型肝炎, 隐匿性/共感染/无偿献血者/HCV基因型/PCRKey words
hepatitis C virus/hepatitis B, occult/co-infection/voluntary blood donors/HCV genotype分类
医药卫生引用本文复制引用
王淏,付涌水,许茹,王敏,黄杰庭,廖峭,单振刚,钟惠珊,郑优荣,戎霞..无偿献血人群中HCV/OBI共感染者的分子流行病学特征[J].中国输血杂志,2019,32(2):123-127,5.基金项目
国家自然科学基金(81772208) （81772208）
广东省医学科学技术研究基金(B2017109) （B2017109）
广州市医学重点学科建设项目(血液安全重点实验室)(穗卫科教[2016]27号) （血液安全重点实验室）
