中国实用外科杂志2019，Vol.39Issue(4)：361-364,4.DOI:10.19538/j.cjps.issn1005-2208.2019.04.18
Ⅱ～Ⅲ期结直肠癌病人围手术期外周血循环肿瘤细胞检测及其临床意义
Detection and clinical significance of perioperative circulating tumor cells in patients with stage Ⅱ to Ⅲ colorectal carcinoma
摘要
Abstract
Objective To detect perioperative circulating tumor cells (CTCs) in patients with colorectal carcinoma and evaluate their clinical significance. Methods A total of 69 patients with stage Ⅱ to Ⅲ sporadic primary colorectal cancer were collected from Department of General Surgery, Beijing Friendship Hospital, Capital Medical University from June 2015 to August 2017. All the patients received surgery and no preoperative neoadjuvant therapy. Peripheral blood samples were collected before operation (Pre-OP), on three days post-operation (POD3), and one 14 days post-operation (POD 14). CTCs were detected using density-gradient centrifugation and immunofluorescence staining. Associations of perioperative CTCs with clinicopathological features and tumor recurrence were determined. Results Average Pre-OP, POD3, and POD14 CTCs were (3.8 ± 0.5) number/3.5 mL, (6.6 ± 1.4) number/3.5 mL, and (3.5 ± 0.5) number/3.5 mL, respectively. There was significant difference between POD3 and POD14 CTCs (P<0.05). Pearson correlation analysis suggested that Pre-OP CTCs was significantly associated with POD14 CTCs (r=0.433, P<0.001). Association of POD14 CTCswith TNM stage was found (χ2=3.916, P=0.048) and there was a trend of association between POD3 CTCs with gender (χ2=3.857, P=0.050). The followup duration was 15.6 (3.5-35.0) months and the recurrence rate of the patients was 10.6％ (7/63). Kaplan-Meier curves revealed no significant associations oftumor recurrence with POD3 CTCs (P=0.194) and POD14 CTCs (P=0.269). Conclusion Compared with preoperative CTCs, postoperative CTCs are firstly increased and then decreased. The postoperative CTCs are potential biomarkers for colorectal cancer recurrence but needed to be verified in more studies.关键词
结直肠癌/循环肿瘤细胞/肿瘤复发Key words
colorectal carcinoma/circulating tumor cells/tumor recurrence分类
医药卫生引用本文复制引用
汪栋,金岚,王今,白志刚,王婷婷,丁国乾,杨盈赤,姚宏伟,张忠涛..Ⅱ～Ⅲ期结直肠癌病人围手术期外周血循环肿瘤细胞检测及其临床意义[J].中国实用外科杂志,2019,39(4):361-364,4.基金项目
国家科技支撑计划资助项目国家科技支撑计划课题(No.2015BAI13B09) （No.2015BAI13B09）
北京市医管局临床医学发展专项经费资助项目(No.ZYLX201504) （No.ZYLX201504）
北京市卫生系统高层次卫生技术人才培养计划(No.2015-3-005) （No.2015-3-005）
