中国体外循环杂志2019，Vol.17Issue(1)：48-53,6.DOI:10.13498/j.cnki.chin.j.ecc.2019.01.11
四氢姜黄素对H9c2细胞缺血再灌注损伤的影响及机制研究
Protective effects of tetrahydrocurcum on myocardial ischemia reperfusion injury of H9c2 cells
摘要
Abstract
Objective To investigate the protective effects and underlying mechanisms of tetrahydrocurcumin (THC) pretreatment on ischemia reperfusion injury (IRI) of H9c2 cells. Methods To mimic the in vivo ischemia reperfusion model, H9c2 cells were exposed to an ischemic buffer for 1 h followed by serum-free DMEM for 4 h. Prior to this procedure, cells were treated with or without THC. They were divided into four groups: Control group, THC group, SIR group and SIR+THC group, respectively. Four hours after the reperfusion, cell viability, reactive oxygen species (ROS) production, malonaldehyde (MDA) content, activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) as well as the expression levels of Bax, Bcl-2 and Caspase-3 were measured in each group. Results Compared with SIR group, THC pretreatment increased the cell viability, the expression of Bcl-2 and the activities of SOD and GSH-Px in H9c2 cells. In addition, THC also significantly decreased the apoptotic ratio, the expression of Bax and Caspase-3, ROS production and MDA content. Conclusion THC pretreatment could effectively attenuate myocardial IRI by reducing SIR-induced oxidative stress and apoptosis.关键词
四氢姜黄素/细胞/缺血再灌注损伤/凋亡/氧化应激/预处理Key words
Tetrahydrocurcumin/Cell/Myocardial ischemia reperfusion injury/Apoptosis/Oxidative stress/Pretreatment引用本文复制引用
冯建梅,冯建宇,吴岩,翟蒙恩,冯笑,金振晓,曹磊..四氢姜黄素对H9c2细胞缺血再灌注损伤的影响及机制研究[J].中国体外循环杂志,2019,17(1):48-53,6.基金项目
国家自然科学基金资助项目 (81570231) （81570231）
