中国畜牧兽医2019，Vol.46Issue(1)：1-9,9.DOI:10.16431/j.cnki.1671-7236.2019.01.001
3个时期牛肌肉组织中长链非编码RNA表达谱的生物信息学分析
Bioinformatics Analysis of Long Non-coding RNA Expression Profiles in Bovine Muscle Tissues from Three Periods
摘要
Abstract
This study was aimed to analyze the expression profiles of long non-coding RNA (lncRNAs) in bovine muscle tissue from different periods and screen out lncRNAs which might involve in muscle development.The bovine muscle tissue RNA samples from 3-month-old embryos, 6-month-old embryos and 9-month-old calf were collected for high-throughput sequencing.The identified lncRNAs were analyzed and filtered with bioinformatics methods, Veen analysis was performed on lncRNAs target gene and three periods differential mRNAs, Go and KEGG enrichment analysis were performed on Veen results to select lncRNAs and target mRNAs which related to muscle development.The results showed that 212 851 novel unannotated lncRNAs were identified by high-throughput sequencing, of which 9 913 were differentially expressed in differentperiods.These lncRNAs distributed on each chromosome and belonged to four types, which included intergenic, sense strand, antisense strand and bidirectional, and the sense strand lncRNAs had the largest number and the widest distribution range on each chromosome.The structural features analysis revealed that these lncRNAs had the characteristics of short open reading frame, low expression level, weak coding ability, wide distribution and the huge numbers.55 muscle myogenesis related differentially expressed lncRNAs among 3-month-old embryos, 6-month-old embryos and 9-month-old calf were screened out by GO and KEGG enrichment analysis, and 38 candidate target mRNAs were also filtered.This study presented the expression pattern of lncRNAs in bovine muscle tissue from three periods by predicting the interaction between lncRNAs and mRNA, and greatly reduced the research range of bovine muscle development related lncRNAs.The study also provided a critical target for revealing the function of bovine muscle development.关键词
牛肌肉组织/RNA测序/生物信息学分析/lncRNAsKey words
bovine muscle tissue/RNA sequencing/bioinformatics analysis/lncRNAs分类
生物科学引用本文复制引用
张晓娟,陈明明,辛向博,刘新峰,张林林,丁向彬,郭宏,李新..3个时期牛肌肉组织中长链非编码RNA表达谱的生物信息学分析[J].中国畜牧兽医,2019,46(1):1-9,9.基金项目
国家自然科学基金(31501939) （31501939）
天津市自然科学基金(16JCZDJC33300) （16JCZDJC33300）
