中国畜牧兽医2019，Vol.46Issue(1)：20-27,8.DOI:10.16431/j.cnki.1671-7236.2019.01.003
成纤维细胞生长因子21及其受体1、2在小鼠毛囊形成期的定位和表达
Localization and Expression of FGF21, FGFR1 and FGFR2 in Mice Hair Follicle Formation
摘要
Abstract
This study had investigated the localization and expression of fibroblast growth factor21 (FGF21) with its receptors FGFR1 and FGFR2in the formation phase of embryonic mice hair follicles.E13-E18 embryonic mice back skin were used to determine the mRNA and protein relative expression levels of FGF21, FGFR1 and FGFR2by immunohistochemistry, Real-time PCR and Western blotting analysis.The results showed that: (1) FGF21expressed in epidermis, basal layer and connective tissue at E13;Expressed in epidermis and placode at E14;Slightly expressed in hair bud at E15;Expressed in hair peg at E16 and E17;Mainly expressed in immature hair follicle at E18.FGFR1 and FGFR2expressed in epidermis, basal layer, placode, hair bud, hair peg, immature hair follicle and connective tissue at E13-E18. (2) The results of Real-time PCR and Western blotting showed that the relative expression levels of FGF21 mRNA and protein were higher at E14 than that of any other stages;The relative expression levels of FGFR1 mRNA and protein were higher at E16-E17;And the relative expression of FGFR2 mRNA and protein showed as-cending trend from E13 to E18and was highest at E18.In summary, during the embryonic hair follicle formation and development, FGF21 might play a role in inducing hair follicle activation;FGFR1might promote hair peg formation;FGFR2might be necessary to induce hair follicle formation and hair follicle maturation.关键词
成纤维细胞生长因子21 (FGF21)/FGFR1/FGFR2/小鼠胚胎/毛囊形成期Key words
FGF21/FGFR1/FGFR2/embryonic mice/hair follicle formation stage分类
生物科学引用本文复制引用
陆娜,曹校瑞,吴晋强,闫瑞琴,张娇娇,吴佳豪,赫晓燕..成纤维细胞生长因子21及其受体1、2在小鼠毛囊形成期的定位和表达[J].中国畜牧兽医,2019,46(1):20-27,8.基金项目
山西省自然科学基金(201701D121106) （201701D121106）
