中国畜牧兽医2019，Vol.46Issue(1)：89-100,12.DOI:10.16431/j.cnki.1671-7236.2019.01.011
活性酵母添加对饲喂不同精粗比日粮肉牛血浆代谢组的影响
Effect of Saccharomyces cerevisiae on Plasma Metabolomics of Steers Fed Diets with Different Concentrate to Forage Ratios
摘要
Abstract
The objective was to assess the effects of Saccharomyces cerevisiae (SC) supplementation on plasma metabolomics of steers fed diets with different concentrate to forage ratios (CTFR) .10Simental×Local breed steers (450kg±50kg) fitted with rumen fistulas were assigned to control and treatment groups.Steers in the two groups were fed the same basal diets but steers in treatment group received supplementation with SC (8×109 CFU/h per day through the ruminal fistula) following a 2-period crossover design.Each period consisted of four phases, each of which lasted for 17 d, 16dfor dietary adaptation, and 1dfor rumen sample collection.From the 1st tothe 4th phase, steers were fed in a stepwise fashion with increasing CTFRs (30∶70, 50∶50, 70∶30, 90∶10) .UPLC-QTOF-MS was used to detected plasma metabolomics.PCA, PLS-DA and OPLS-DA were used to filtrate biomarkers.The results showed that biomarkers among different dietary CTFR groups were cytosine, dihydrothymine, palmitic amide, stearamide, oleamide and D-4-phosphopantothenate.When dietary CTFR was 30∶70, biomarkers between SC supplementation and control groups were sphinganine, phytosphingosine and PC (18∶0) ;When CTFR was50∶50, biomarkers between the two groups were 13E-docosenamide, 1-monoacylglycerol and PC (16∶0) .When dietary CTFR was 70∶30, biomarkers were 1-monoacylglycerol, diacylglycerol, triacylglycerol and PC (18∶0, 17∶0) , while the biomarkers were 13E-docosenamide, glycocholic acid and PC (18∶2, 20∶2) when CTFR was 90∶10.The results showed that with concentrate level increasing, fat metabolism was improved, but rumen wall was damaged;SC supplementation could improve phospholipid and fat metabolism, which was beneficial to maintain animal health status and improve production performance.关键词
活性酵母/血浆代谢组/肉牛/精粗比Key words
live yeast/plasma metabolomics/beef cattle/concentrate to forage ratio (CTFR)分类
农业科技引用本文复制引用
李岩,丁耿芝,姚倩倩,吴浩,孟庆翔,周振明..活性酵母添加对饲喂不同精粗比日粮肉牛血浆代谢组的影响[J].中国畜牧兽医,2019,46(1):89-100,12.基金项目
优质肉牛高效安全养殖技术应用与示范(2018YFD0501700) （2018YFD0501700）
公益性行业(农业)科研专项经费项目(201503134) （农业）
国家肉牛牦牛产业技术体系项目(CARS-37) （CARS-37）
