中国畜牧兽医2019，Vol.46Issue(1)：194-199,6.DOI:10.16431/j.cnki.1671-7236.2019.01.022
哺乳动物体内、外生产胚胎的表观遗传差异研究进展
Research Progress on Epigenetic Differences Between Mammalian Embryos Produced in vivo and in vitro
摘要
Abstract
As an efficient assisted reproductive technology, in vitro embryo production is of great significance to the conservation and effective utilization of germplasm resources and genetic improvement.However, compared with embryos produced in vivo, the embryos produced in vitro develop slowly, the cleavage rate is low and the rate of apoptosis increases in the process of culture, which are accompanied by placental hypertrophy and prolonged pregnancy after transplantation.Preterm delivery, low birth weight, and macrosomia syndrome (LOS) occur after birth which are closely related to abnormal gene expression and epigenetic modification.The progress of epigenetic modification in the production of embryos in vivo and in vitro was briefly reviewed in this paper.The differences of epigenetic modification of imprinted genes, DNA methylation and histone acetylation between mammalian embryos in vivo and in vitro were introduced.The application of microarray technique in the production of embryos in vitro and in vivo was briefly described in order to obtain the key imprinted genes that lead to the difference of embryo quality betweenin vivoand in vitro, and explored the reasons why the quality of embryos produced in vitro was lower than that of in vivo, and then improved the in vitro embryo production system.关键词
囊胚/甲基化/表观遗传/组蛋白乙酰化Key words
blastocyst/methylation/epigenetics/histone acetylation分类
农业科技引用本文复制引用
赵亚涵,郝海生,杜卫华,闫长亮,刘岩,庞云渭,朱化彬,赵学明..哺乳动物体内、外生产胚胎的表观遗传差异研究进展[J].中国畜牧兽医,2019,46(1):194-199,6.基金项目
中国农业科学院"科研英才培育工程" （）
中国农业科学院科技创新工程(ASTIP-IAS06) （ASTIP-IAS06）
