中国畜牧兽医2019，Vol.46Issue(1)：200-205,6.DOI:10.16431/j.cnki.1671-7236.2019.01.023
猪miR-23a靶向调控Smad3基因表达的研究
Study on Regulation of Gene Expression of Smad3 Gene by miR-23a in Pig
摘要
Abstract
To determine whether the miR-23 aregulate Smad3gene, wild-type and mutant-type dual-luciferase reporter vectors containing Smad3-3′-UTR (psiCHECKTM-2-W-Smad3-3′-UTR and psiCHECKTM-2-M-Smad3-3′-UTR) were constructed using Not Ⅰ and Xho Ⅰ, miR-23 amimics, miR-23 ainhibitor and their negative control were transfected with or without dual luciferase reporter vectors in PK-15 cells, luciferase activity were assayed, the mRNA and protein expression levels of Smad3 gene were determined by Real-time PCR and Western blotting, respectively.The results showed that the luciferase activity in PK-15 cells transfected with wild-type dual-luciferase reporter vectors and miR-23 amimic was significantly lower than negative control (P<0.05) .In PK-15 cells transfected with miR-23 amimic, the expression levels of Smad3 mRNA and Smad3 protein were significantly down-regulated (P<0.05) .While there was no significant difference in the expression level of Smad3 protein between PK-15 cells tansfected with miR-23 ainhibitor and its negative control (P>0.05) .The results indicated that miR-23 acould regulate directely the expression of its target gene Smad3.关键词
miR-23a/Smad3基因/表达调控Key words
miR-23a/Smad3 gene/expression regulation分类
农业科技引用本文复制引用
邱梅玉,翟腾蛟,黄涛,李涛,孙敬礼,孙晓梅..猪miR-23a靶向调控Smad3基因表达的研究[J].中国畜牧兽医,2019,46(1):200-205,6.基金项目
国家自然科学基金(31460586) （31460586）
