中国畜牧兽医2019，Vol.46Issue(1)：223-230,8.DOI:10.16431/j.cnki.1671-7236.2019.01.026
鸭源多杀性巴氏杆菌对磺胺类药物的耐药性检测及耐药基因分析
Detection of Sulfonamides Sensitivity and Analysis of Resistant Genes of Pasteurella multocida Isolated from Ducks
摘要
Abstract
In order to understand the antimicrobial sensitibity to sulfonamides and distribution ofsulfonamides resistance genes of Pasteurella multocida strains isolated from ducks in Fujian, Guangdong, Jiangxi, etc, 89 Pasteurella multocida strains were tested for antimicrobial susceptibility to trimethoprim-sulfamethoxazole by agar dilution method, PCR method and multi-locus sequence typing (MLST) were used for detection of sulfonamides resistance genes and to analyze the genetic relationship among the sulfonamides-resistant strains.The results showed that 33strains (37.1％) were resistant to trimethoprim-sulfamethoxazole.Among the 33 sulfonamidesresistant strains, the detection rates of sul1, sul2 and sul3genes were 100％ (33/33) , 97.0％ (32/33) and 21.2％ (7/33) .The simultaneous detection rate of sul1 and sul2genes were 97.0％ (32/33) .The simultaneous detection rate of sul1, sul2 and sul3genes were 21.2％ (7/33) .All of the33sulfonamides-resistant strains belonged to the ST129 sequence type.The results indicated that there was sulfonamides resistance to Pasteurella multocidaisolated from ducks, and all the sulfonamides-resistant strains, which belonged to the same sequence type ST129, carried sul1 resistant gene, most of sulfonamides-resistant strains carried sul1 and sul2resistant genes, suggesting that it should be rationally used sulfonamides to prevent and cure the infection of Pasteurella multocidaisolated from ducks in some areas of Fujian, Guangdong and Jiangxi, etc.关键词
多杀性巴氏杆菌/磺胺类药物/多位点序列分型/sul1基因/sul2基因/sul3基因Key words
Pasteurella multocida/sulfonamides/MLST/sul1 gene/sul2 gene/sul3 gene分类
农业科技引用本文复制引用
陈红梅,黄瑜,黄小红,程龙飞,邓辉,刘荣昌,傅光华,施少华,傅秋玲,万春和,郑庆礼..鸭源多杀性巴氏杆菌对磺胺类药物的耐药性检测及耐药基因分析[J].中国畜牧兽医,2019,46(1):223-230,8.基金项目
现代农业产业技术体系建设专项资金(CARS-42-18) （CARS-42-18）
福建省属公益类科研院所基本科研项目(2016R1022-2) （2016R1022-2）
福建省农业科学院畜牧兽医创新团队(STIT2017-1-5) （STIT2017-1-5）
福建省教育厅实验室开放基金项目 （）
