中国畜牧兽医2019，Vol.46Issue(1)：231-238,8.DOI:10.16431/j.cnki.1671-7236.2019.01.027
马源马链球菌兽疫亚种新疆株的分离鉴定及遗传特性分析
Isolation, Identification and Genetic Characterization Analysis of Streptococcus equi subsp. zooepidemicus from Horse in Xinjiang
摘要
Abstract
To understand the molecular evolution and mutation of SzM gene of Streptococcus equi subsp.zooepidemicus (S.zooepidemicus) from horse in Xinjiang and provide basis for the prevention and treatment of infectious disease, lymph gland samples from sick horse were collected for bacterium culture, biochemical identification, and drug sensitivity test was also tested.The SzM gene was amplified from genomic DNA of S.zooepidemicus isolate by PCR based on the primers designed according to the published sequence in GenBank.Then the amplified SzM gene was cloned, sequenced and analyzed, and phylogenetic tree was constructed.The results showed that the isolate was Gram-positive Streptococcus, which was named as S.zooepidemicus ZMSY15-1.Drug sensitivity test showed that S.zooepidemicus ZMSY15-1was resistant to penicillin and sul-fadiazine sodium salt, and sensitive to other 14 microbials.The amino acid homologies of SzM gene of ZMSY15-1with different strains at home and abroad were 56.0％to 70.0％.In addition, the phylogenetic analysis indicated that these test strains belonged to 4different clades, the ZMSY15-1isolate shared identities of 59.9％ with swine isolates and they belonged to two different groups.The ZMSY15-1isolate was closely related to US horse NH55426.The results could enrich the information data of SzMgene of S.zooepidemicusin domestic horses, and provide experimental data for the pathogenic mechanism and prevention control of S.zooepidemicus.关键词
马链球菌兽疫亚种/分离鉴定/SzM基因/序列分析Key words
S. zooepidemicusi/isolation and identification/SzM gene/sequence analysis分类
农业科技引用本文复制引用
冯凯,王浩,周婷婷,马晓慧,恩克·博力德,佘明江,苏艳..马源马链球菌兽疫亚种新疆株的分离鉴定及遗传特性分析[J].中国畜牧兽医,2019,46(1):231-238,8.基金项目
国家自然科学基金(31660707) （31660707）
新疆维吾尔自治区重大科技专项项目(2017A01002-2-6) （2017A01002-2-6）
