中国畜牧兽医2019，Vol.46Issue(1)：264-270,7.DOI:10.16431/j.cnki.1671-7236.2019.01.031
杂交鲟类志贺邻单胞菌的分离鉴定及耐药性分析
Isolation, Identification and Drug Resistance Analysis of Plesiomonas shigelloides from Hybrid Surgeon
摘要
Abstract
This paper was aimed to study on isolation, identification and drug resistance analysis of a bacterial (NZ-2017) , which was isolated from local sick hybrid sturgeons.Morphology, physiological and biochemical characteristics and phylogenetic analysis of 16 SrRNA gene were used to identify its species.The filter paper diffusion method test, antimicrobial resistance genes test and animal regression test were carried out to analyze its pathogenicity.The results showed that NZ-2017 was a gram-negative bacillus, which was 99.70％ homologous with Plesiomonas shigelloides16 SrRNA gene fragment by sequencing and BLAST analysis.With the results of physiological and biochemical identification, NZ-2017 was confirmed as Plesiomonas shigelloides.The drug sensitivity test results showed that NZ-2017 was sensitive to ofloxacin, norfloxacin, ciprofloxacin, doxycycline, and resistance to kanamycin, amoxicillin, tylosin and sulfamethoxazole.The antimi-crobial resistance genes test results showed that there were three antimicrobial resistance genes (Sul1, Sul2 and Intl1) , which was compatible with phenotype.The animal regression test result showed that NZ-2017 had pathogenicity to mouse.This study conducted an accurate diagnosis of the cause of the epidemic of sturgeons in Guizhou, and provided a basis for the prevention and controls to this bacterial disease of fish in this region.关键词
杂交鲟/类志贺邻单胞菌/分离鉴定/16SrRNA基因/药敏试验/耐药基因Key words
hybrid sturgeon/Plesiomonas shigelloides/isolation and identification/16S rRNA gene/antibiotic sensitivity test/antimicrobial resistance genes分类
农业科技引用本文复制引用
张明洋,胡安东,程振涛,姜海波,文明..杂交鲟类志贺邻单胞菌的分离鉴定及耐药性分析[J].中国畜牧兽医,2019,46(1):264-270,7.基金项目
贵州省科技支撑计划项目(黔科合支撑[2016]2502号、黔科合支撑[2016]2511号、黔科合支撑[2017]2532号) （黔科合支撑[2016]2502号、黔科合支撑[2016]2511号、黔科合支撑[2017]2532号）
贵州省科技创新人才团队建设项目(黔科合人才团队[2015]4016号) （黔科合人才团队[2015]4016号）
